Gain of function mutations of RTK conserved residues display differential effects on NTRK1 kinase activity

Claudia Miranda, Giuseppe Zanotti, Sonia Pagliardini, Carola Ponzetto, Marco A. Pierotti, Angela Greco

Research output: Contribution to journalArticlepeer-review

Abstract

Activation of tyrosine kinase receptors is associated with human tumors. Tumorigenic versions of several RTKs, such as Ret, Kit and Met carry activating mutations at highly conserved residues of the tyrosine kinase domain. We have investigated the effect of some of these mutations on the NTRK1/NGF receptor, for which no naturally occurring activating point mutations have been so far detected. We introduced the following mutations in NTRK1 tyrosine kinase domain: (i) D668N equivalent to Met D1246N associated to HPRC; (ii) D668V modelled on Kit D816V found in mastocytosis; (iii) M688T corresponding to Ret M918T associated to the cancer syndrome MEN2B. The Met-like mutation rendered the NTRK1 receptor more responsive to ligand, as observed for the corresponding mutation in Met. On the contrary the Kit-like D668V resulted as neutral mutation. Surprisingly, the MEN2B-like M688T completely abrogated NTRK1 receptor activity, resulting as a loss of function mutation. Our results show that the mutations tested, although involving conserved amino acids in highly homologous regions, exert distinct effects in different receptors, and suggest a very peculiar auto-inhibitory mechanism for NTRK1.

Original languageEnglish
Pages (from-to)8334-8339
Number of pages6
JournalOncogene
Volume21
Issue number54
DOIs
Publication statusPublished - Nov 28 2002

Keywords

  • NTRK1
  • Oncogenic activation
  • Tyrosine kinase receptors

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Gain of function mutations of RTK conserved residues display differential effects on NTRK1 kinase activity'. Together they form a unique fingerprint.

Cite this