Galanin reduces PDBu-induced protein phosphorylation in rat ventral hippocampus

Caterina La Porta, Roberto Bianchi, Silvano Sozzani, Tamas Bartfai, Silvana Consolo

Research output: Contribution to journalArticlepeer-review

Abstract

The effect of galanin(GAL) on basal and phorbol-12,13-dibutyrate (PDBu) induced protein phosphorylation in rat ventral hippocampal miniprisms was investigated. GAL (0.5, 1 and 2μM) inhibited PDBu stimulation in a concentration-dependent manner without altering basal protein phosphorylation. This inhibitory effect was prevented by the GAL antagonist galantide. GAL did not affect either the activity of protein kinase C (PKC) from rat brain or basal phosphorylation in ventral hippocampal hippogenates, suggesting that it did not directly modulate PKC activity, Depolarization of miniprisms from ventral hippocampl by 18 mM K+ prevented the effect of GAL on PDBu-induced phosphorylation. The results indicate that GAL indirectly regulates neuronal protein phosphorylation by a GAL receptor-mediated action.

Original languageEnglish
Pages (from-to)46-48
Number of pages3
JournalFEBS Letters
Volume300
Issue number1
DOIs
Publication statusPublished - Mar 23 1992

Keywords

  • Galanin antagonist
  • Neuropeptide
  • Protein kinase C
  • Second messenger

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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