Galectin-4 controls intestinal inflammation by selective regulation of peripheral and mucosal T cell apoptosis and cell cycle

Daniela Paclik, Silvio Danese, Uta Berndt, Bertram Wiedenmann, Axel Dignass, Adreas Sturm

Research output: Contribution to journalArticle

Abstract

Galectin-4 is a carbohydrate-binding protein belonging to the galectin family. Here we provide novel evidence that galectin-4 is selectively expressed and secreted by intestinal epithelial cells and binds potently to activated peripheral and mucosal lamina propria T-cells at the CD3 epitope. The carbohydrate-dependent binding of galectin-4 at the CD3 epitope is fully functional and inhibited T cell activation, cycling and expansion. Galectin-4 induced apoptosis of activated peripheral and mucosal lamina propria T cells via calpain-, but not caspase-dependent, pathways. Providing further evidence for its important role in regulating T cell function, galectin-4 blockade by antisense oligonucleotides reduced TNF-alpha inhibitor induced T cell death. Furthermore, in T cells, galectin-4 reduced pro-inflammatory cytokine secretion including IL-17. In a model of experimental colitis, galectin-4 ameliorated mucosal inflammation, induced apoptosis of mucosal T-cells and decreased the secretion of pro-inflammatory cytokines. Our results show that galectin-4 plays a unique role in the intestine and assign a novel role of this protein in controlling intestinal inflammation by a selective induction of T cell apoptosis and cell cycle restriction. Conclusively, after defining its biological role, we propose Galectin-4 is a novel anti-inflammatory agent that could be therapeutically effective in diseases with a disturbed T cell expansion and apoptosis such as inflammatory bowel disease.

Original languageEnglish
Article numbere2629
JournalPLoS One
Volume3
Issue number7
DOIs
Publication statusPublished - Jul 9 2008

Fingerprint

Galectin 4
T-cells
cell cycle
Cell Cycle
apoptosis
T-lymphocytes
inflammation
Cells
Apoptosis
Inflammation
T-Lymphocytes
epitopes
Epitopes
Mucous Membrane
cytokines
secretion
Cytokines
Galectins
anti-inflammatory agents
carbohydrate binding

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Galectin-4 controls intestinal inflammation by selective regulation of peripheral and mucosal T cell apoptosis and cell cycle. / Paclik, Daniela; Danese, Silvio; Berndt, Uta; Wiedenmann, Bertram; Dignass, Axel; Sturm, Adreas.

In: PLoS One, Vol. 3, No. 7, e2629, 09.07.2008.

Research output: Contribution to journalArticle

Paclik, Daniela ; Danese, Silvio ; Berndt, Uta ; Wiedenmann, Bertram ; Dignass, Axel ; Sturm, Adreas. / Galectin-4 controls intestinal inflammation by selective regulation of peripheral and mucosal T cell apoptosis and cell cycle. In: PLoS One. 2008 ; Vol. 3, No. 7.
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