TY - JOUR
T1 - Ganglioside content of human platelets - Differences in resting and activated platelets
AU - Ferroni, Patrizia
AU - Lenti, Luisa
AU - Martini, Francesca
AU - Ciatti, Filippo
AU - Pontieri, Giuseppe M.
AU - Gazzaniga, Pier Paolo
PY - 1997/3
Y1 - 1997/3
N2 - Gangliosides may play functional roles in platelet physiology, therefore this study has been designed to evaluate whether changes in ganglioside composition may occur as a consequence of platelet activation. The results obtained indicate that lactosylceramide and GM3 are the major glycosphingolipids of human platelets. The lipid-bound sialic acid (LBSA) content was 1.27 ± 0.04 μg/mg of protein. Resting platelets did not express GD3; GD3 was synthesized upon platelet activation (24 ± 8 ng/mg of protein). The stimulation of platelets with adenosine diphosphate showed the appearance of GD3 even in the absence of degranulation. Finally, incorporation of pyrene-labeled GM3 into platelet membranes, followed by stimulation with adenosine diphosphate, resulted in the appearance of a fluorescent band comigrating with GD3. The present studies indicate that sialyltransferase activation may occur as an early event following platelet stimulation, leading to GD3 synthesis mainly from the GM3 pool.
AB - Gangliosides may play functional roles in platelet physiology, therefore this study has been designed to evaluate whether changes in ganglioside composition may occur as a consequence of platelet activation. The results obtained indicate that lactosylceramide and GM3 are the major glycosphingolipids of human platelets. The lipid-bound sialic acid (LBSA) content was 1.27 ± 0.04 μg/mg of protein. Resting platelets did not express GD3; GD3 was synthesized upon platelet activation (24 ± 8 ng/mg of protein). The stimulation of platelets with adenosine diphosphate showed the appearance of GD3 even in the absence of degranulation. Finally, incorporation of pyrene-labeled GM3 into platelet membranes, followed by stimulation with adenosine diphosphate, resulted in the appearance of a fluorescent band comigrating with GD3. The present studies indicate that sialyltransferase activation may occur as an early event following platelet stimulation, leading to GD3 synthesis mainly from the GM3 pool.
UR - http://www.scopus.com/inward/record.url?scp=0030899641&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030899641&partnerID=8YFLogxK
M3 - Article
C2 - 9066009
AN - SCOPUS:0030899641
VL - 77
SP - 548
EP - 554
JO - Thrombosis and Haemostasis
JF - Thrombosis and Haemostasis
SN - 0340-6245
IS - 3
ER -