Gangliosides prevent the inhibition by K-252a of NGF responses in PC12 cells

G. Ferrari, M. Fabris, M. G. Fiori, N. Gabellini, C. Volontè

Research output: Contribution to journalArticlepeer-review

Abstract

K-252a, a general kinase inhibitor, selectively blocks the actions of nerve growth factor (NGF) in PC12 cells. Since gangliosides have been reported to modulate neuronal cell responsiveness to NGF and to regulate several protein kinases, the ability of these compounds to reverse the inhibition by K-252a was tested. Parameters at both short- and long-term times following treatment of PC12 cells with NGF were analyzed which are known to be either transcription-dependent or -independent events. Gangliosides were found to completely prevent the inhibition by K-252a of NGF-induced neurite regeneration and c-fos induction, and partially also that of protein kinase N activation. The ganglioside protective effects were concentration-dependent and required the intact molecule. These findings raise the possibility that gangliosides might affect a specific pathway of NGF responses sensitive to inhibition by K-252a.

Original languageEnglish
Pages (from-to)35-42
Number of pages8
JournalDevelopmental Brain Research
Volume65
Issue number1
DOIs
Publication statusPublished - Jan 17 1992

Keywords

  • Ganglioside
  • K-252a
  • Nerve growth factor
  • PC12
  • Protein kinase

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Fingerprint

Dive into the research topics of 'Gangliosides prevent the inhibition by K-252a of NGF responses in PC12 cells'. Together they form a unique fingerprint.

Cite this