Gas chromatographic determination of hexamethylmelamine in mouse plasma

Maurizio D'Incalci, Paolo Morazzoni, Claudio Pantarotto

Research output: Contribution to journalArticlepeer-review

Abstract

A sensitive and specific method involving gas chromatographic separation with nitrogen-phosphorus selective detection has been developed and applied for the determination of hemamethylmelamine in mouse plasma. Following extraction with n-hexane, the drug is quantitatively evaluated using triethylenemelamine as external standard. The lower limit of sensitivity of the method is 10 ng/ml of mouse plasma and detector response was linear up to 2 μg/ml. The plasma disappearance curve after a single intraperitoneal dose of hexamethylmelamine to mice is reported. Pentamethylmelamine and N2,N2,N4,N6-tetramethylmelamine were identified in mouse plasma as hexamethylmelamine metabolites by combined gas chromatography-mass spectrometry.

Original languageEnglish
Pages (from-to)441-449
Number of pages9
JournalAnalytical Biochemistry
Volume99
Issue number2
DOIs
Publication statusPublished - Nov 1 1979

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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