Gas chromatographic-mass spectrometric determination of intact C3-hydroxylated benzodiazepine glucuronides in urine

F. Marcucci, R. Bianchi, L. Airoldi, M. Salmona, R. Fanelli, C. Chiabrando, A. Frigerio, E. Mussini, S. Garattini

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A method is described for the determination of C3-hydroxylated benzodiazepine glucuronides in biological samples. Oxazepam and lorazepam glucuronides are measured as methyl esters and trimethylsilyl derivatives by a gas chromatographic procedure. The applicability of the method has been tested on the urine of rats, guinea pigs, rabbits and man, receiving oxazepam orally. Oxazepam glucuronide was not found in rat urine but it was present in the urine of rabbits (5.7% of the administered dose), guinea pigs (9.5 %) and man (13.4-26.9%).

Original languageEnglish
Pages (from-to)285-293
Number of pages9
JournalJournal of Chromatography A
Volume107
Issue number2
DOIs
Publication statusPublished - Apr 16 1975

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Glucuronides
Benzodiazepines
Rats
Gases
Urine
Oxazepam
Guinea Pigs
Rabbits
Esters
Derivatives
oxazepam glucuronide
lorazepam glucuronide

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Gas chromatographic-mass spectrometric determination of intact C3-hydroxylated benzodiazepine glucuronides in urine. / Marcucci, F.; Bianchi, R.; Airoldi, L.; Salmona, M.; Fanelli, R.; Chiabrando, C.; Frigerio, A.; Mussini, E.; Garattini, S.

In: Journal of Chromatography A, Vol. 107, No. 2, 16.04.1975, p. 285-293.

Research output: Contribution to journalArticle

Marcucci, F. ; Bianchi, R. ; Airoldi, L. ; Salmona, M. ; Fanelli, R. ; Chiabrando, C. ; Frigerio, A. ; Mussini, E. ; Garattini, S. / Gas chromatographic-mass spectrometric determination of intact C3-hydroxylated benzodiazepine glucuronides in urine. In: Journal of Chromatography A. 1975 ; Vol. 107, No. 2. pp. 285-293.
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