Gas chromatographic-mass spectrometric determination of intact C3-hydroxylated benzodiazepine glucuronides in urine

F. Marcucci, R. Bianchi, L. Airoldi, M. Salmona, R. Fanelli, C. Chiabrando, A. Frigerio, E. Mussini, S. Garattini

Research output: Contribution to journalArticlepeer-review

Abstract

A method is described for the determination of C3-hydroxylated benzodiazepine glucuronides in biological samples. Oxazepam and lorazepam glucuronides are measured as methyl esters and trimethylsilyl derivatives by a gas chromatographic procedure. The applicability of the method has been tested on the urine of rats, guinea pigs, rabbits and man, receiving oxazepam orally. Oxazepam glucuronide was not found in rat urine but it was present in the urine of rabbits (5.7% of the administered dose), guinea pigs (9.5 %) and man (13.4-26.9%).

Original languageEnglish
Pages (from-to)285-293
Number of pages9
JournalJournal of Chromatography A
Volume107
Issue number2
DOIs
Publication statusPublished - Apr 16 1975

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Fingerprint Dive into the research topics of 'Gas chromatographic-mass spectrometric determination of intact C3-hydroxylated benzodiazepine glucuronides in urine'. Together they form a unique fingerprint.

Cite this