Gas chromatography-mass spectrometry analysis of endogenous cannabinoids in healthy and tumoral human brain and human cells in culture

Mauro Maccarrone, Marina Attinà, Antonella Cartoni, Monica Bari, Alessandro Finazzi-Agrò

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Endocannabinoids are lipid mediators thought to modulate central and peripheral neural functions. We report here gas chromatography-electron impact mass spectrometry analysis of human brain, showing that lipid extracts contain anandamide and 2-arachidonoylglycerol (2-AG), the most active endocannabinoids known to date. Human brain also contained the endocannabinoid-like compounds N-oleoylethanolamine, N-palmitoylethanolamine and N-stearoylethanolamine. Anandamide and 2-AG (0.16 ± 0.05 and 0.10 ± 0.05 nmol/mg protein, respectively) represented 7.7% and 4.8% of total endocannabinoid-like compounds, respectively, N-Palmitoyethanolamine was the most abundant (50%), followed by N-oleoyl (23.6%) and N-stearoyl (13.9%) ethanolamines. A similar composition in endocannabinoid-like compounds was found in human neuroblastoma CHP100 and lymphoma U937 cells, and also in rat brain. Remarkably, human meningioma specimens showed an approximately six-fold smaller content of all N-acylethanolamines, but not of 2-AG, and a similar decrease was observed in a human glioblastoma. These ex vivo results fully support the purported roles of endocannabinoids in the nervous system.

Original languageEnglish
Pages (from-to)594-601
Number of pages8
JournalJournal of Neurochemistry
Volume76
Issue number2
DOIs
Publication statusPublished - 2001

Fingerprint

Endocannabinoids
Cannabinoids
Cell culture
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Brain
Cell Culture Techniques
Cells
Ethanolamines
Lipids
U937 Cells
Neurology
Meningioma
Glioblastoma
Neuroblastoma
Gas Chromatography
Nervous System
Rats
Lymphoma

Keywords

  • 2-arachidonoylglycerol
  • Anandamide
  • Endocannabinoids
  • Human brain
  • Neurotoxicity

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Gas chromatography-mass spectrometry analysis of endogenous cannabinoids in healthy and tumoral human brain and human cells in culture. / Maccarrone, Mauro; Attinà, Marina; Cartoni, Antonella; Bari, Monica; Finazzi-Agrò, Alessandro.

In: Journal of Neurochemistry, Vol. 76, No. 2, 2001, p. 594-601.

Research output: Contribution to journalArticle

Maccarrone, Mauro ; Attinà, Marina ; Cartoni, Antonella ; Bari, Monica ; Finazzi-Agrò, Alessandro. / Gas chromatography-mass spectrometry analysis of endogenous cannabinoids in healthy and tumoral human brain and human cells in culture. In: Journal of Neurochemistry. 2001 ; Vol. 76, No. 2. pp. 594-601.
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