Gas chromatography-mass spectrometry determination of ethylenethiourea hemoglobin adducts: a possible indicator of exposure to ethylene bis dithiocarbamate pesticides

Roberta Pastorelli, Riccardo Allevi, Stefano Romagnano, Giovanna Meli, Roberto Fanelli, Luisa Airoldi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Ethylene bisdithiocarbamates (EBDC) are an important class of fungicides used to control crop diseases and prevent mold. Ethylenethiourea (ETU), reported to be their main degradation and metabolic product in animals and man, may have teratogenic and carcinogenic properties. The feasibility of monitoring exposure to ETU on the basis of the formation of adducts to hemoglobin (Hb) was investigated. Rats given a single oral dose of ETU (from 62.5 to 500 mg/kg body wt) formed stable covalent ETU-Hb adducts. Mild acid hydrolysis of the protein regenerated ETU, allowing its detection by isotope dilution gas chromatography-mass spectrometry (GC-MS). The amount of released ETU increased with the dose. The dose-response curve fitted a linear model only between 62.5 mg/kg and 250 mg/kg. Acid-releasable ETU was also positively identified in the hemoglobin of workers exposed to Mancozeb, an EBDC formulation. In the exposed group, 40% had ETU-Hb adducts levels ranging from 0.5 to 1.42 pmol ETU/mg Hb. Such adducts might be useful for measuring EBDC exposure in humans.

Original languageEnglish
Pages (from-to)306-311
Number of pages6
JournalArchives of Toxicology
Volume69
Issue number5
DOIs
Publication statusPublished - Mar 1995

Fingerprint

Ethylenethiourea
Pesticides
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Hemoglobins
Fungicides
ethylene
Acids
Isotopes
Dilution
Crops
Rats
Hydrolysis
Linear Models
Animals
Fungi

Keywords

  • Adducts
  • Dosimetry
  • Ethylene bisdithiocarbamate fungicides
  • Ethylenethiourea
  • Hemoglobin

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Gas chromatography-mass spectrometry determination of ethylenethiourea hemoglobin adducts : a possible indicator of exposure to ethylene bis dithiocarbamate pesticides. / Pastorelli, Roberta; Allevi, Riccardo; Romagnano, Stefano; Meli, Giovanna; Fanelli, Roberto; Airoldi, Luisa.

In: Archives of Toxicology, Vol. 69, No. 5, 03.1995, p. 306-311.

Research output: Contribution to journalArticle

@article{03ebf4622fd4499f908820ec5ee2c414,
title = "Gas chromatography-mass spectrometry determination of ethylenethiourea hemoglobin adducts: a possible indicator of exposure to ethylene bis dithiocarbamate pesticides",
abstract = "Ethylene bisdithiocarbamates (EBDC) are an important class of fungicides used to control crop diseases and prevent mold. Ethylenethiourea (ETU), reported to be their main degradation and metabolic product in animals and man, may have teratogenic and carcinogenic properties. The feasibility of monitoring exposure to ETU on the basis of the formation of adducts to hemoglobin (Hb) was investigated. Rats given a single oral dose of ETU (from 62.5 to 500 mg/kg body wt) formed stable covalent ETU-Hb adducts. Mild acid hydrolysis of the protein regenerated ETU, allowing its detection by isotope dilution gas chromatography-mass spectrometry (GC-MS). The amount of released ETU increased with the dose. The dose-response curve fitted a linear model only between 62.5 mg/kg and 250 mg/kg. Acid-releasable ETU was also positively identified in the hemoglobin of workers exposed to Mancozeb, an EBDC formulation. In the exposed group, 40{\%} had ETU-Hb adducts levels ranging from 0.5 to 1.42 pmol ETU/mg Hb. Such adducts might be useful for measuring EBDC exposure in humans.",
keywords = "Adducts, Dosimetry, Ethylene bisdithiocarbamate fungicides, Ethylenethiourea, Hemoglobin",
author = "Roberta Pastorelli and Riccardo Allevi and Stefano Romagnano and Giovanna Meli and Roberto Fanelli and Luisa Airoldi",
year = "1995",
month = "3",
doi = "10.1007/s002040050175",
language = "English",
volume = "69",
pages = "306--311",
journal = "Archiv fur Toxikologie",
issn = "0003-9446",
publisher = "Springer Verlag",
number = "5",

}

TY - JOUR

T1 - Gas chromatography-mass spectrometry determination of ethylenethiourea hemoglobin adducts

T2 - a possible indicator of exposure to ethylene bis dithiocarbamate pesticides

AU - Pastorelli, Roberta

AU - Allevi, Riccardo

AU - Romagnano, Stefano

AU - Meli, Giovanna

AU - Fanelli, Roberto

AU - Airoldi, Luisa

PY - 1995/3

Y1 - 1995/3

N2 - Ethylene bisdithiocarbamates (EBDC) are an important class of fungicides used to control crop diseases and prevent mold. Ethylenethiourea (ETU), reported to be their main degradation and metabolic product in animals and man, may have teratogenic and carcinogenic properties. The feasibility of monitoring exposure to ETU on the basis of the formation of adducts to hemoglobin (Hb) was investigated. Rats given a single oral dose of ETU (from 62.5 to 500 mg/kg body wt) formed stable covalent ETU-Hb adducts. Mild acid hydrolysis of the protein regenerated ETU, allowing its detection by isotope dilution gas chromatography-mass spectrometry (GC-MS). The amount of released ETU increased with the dose. The dose-response curve fitted a linear model only between 62.5 mg/kg and 250 mg/kg. Acid-releasable ETU was also positively identified in the hemoglobin of workers exposed to Mancozeb, an EBDC formulation. In the exposed group, 40% had ETU-Hb adducts levels ranging from 0.5 to 1.42 pmol ETU/mg Hb. Such adducts might be useful for measuring EBDC exposure in humans.

AB - Ethylene bisdithiocarbamates (EBDC) are an important class of fungicides used to control crop diseases and prevent mold. Ethylenethiourea (ETU), reported to be their main degradation and metabolic product in animals and man, may have teratogenic and carcinogenic properties. The feasibility of monitoring exposure to ETU on the basis of the formation of adducts to hemoglobin (Hb) was investigated. Rats given a single oral dose of ETU (from 62.5 to 500 mg/kg body wt) formed stable covalent ETU-Hb adducts. Mild acid hydrolysis of the protein regenerated ETU, allowing its detection by isotope dilution gas chromatography-mass spectrometry (GC-MS). The amount of released ETU increased with the dose. The dose-response curve fitted a linear model only between 62.5 mg/kg and 250 mg/kg. Acid-releasable ETU was also positively identified in the hemoglobin of workers exposed to Mancozeb, an EBDC formulation. In the exposed group, 40% had ETU-Hb adducts levels ranging from 0.5 to 1.42 pmol ETU/mg Hb. Such adducts might be useful for measuring EBDC exposure in humans.

KW - Adducts

KW - Dosimetry

KW - Ethylene bisdithiocarbamate fungicides

KW - Ethylenethiourea

KW - Hemoglobin

UR - http://www.scopus.com/inward/record.url?scp=0028985916&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028985916&partnerID=8YFLogxK

U2 - 10.1007/s002040050175

DO - 10.1007/s002040050175

M3 - Article

C2 - 7654134

AN - SCOPUS:0028985916

VL - 69

SP - 306

EP - 311

JO - Archiv fur Toxikologie

JF - Archiv fur Toxikologie

SN - 0003-9446

IS - 5

ER -