Abstract
Ethylene bisdithiocarbamates (EBDC) are an important class of fungicides used to control crop diseases and prevent mold. Ethylenethiourea (ETU), reported to be their main degradation and metabolic product in animals and man, may have teratogenic and carcinogenic properties. The feasibility of monitoring exposure to ETU on the basis of the formation of adducts to hemoglobin (Hb) was investigated. Rats given a single oral dose of ETU (from 62.5 to 500 mg/kg body wt) formed stable covalent ETU-Hb adducts. Mild acid hydrolysis of the protein regenerated ETU, allowing its detection by isotope dilution gas chromatography-mass spectrometry (GC-MS). The amount of released ETU increased with the dose. The dose-response curve fitted a linear model only between 62.5 mg/kg and 250 mg/kg. Acid-releasable ETU was also positively identified in the hemoglobin of workers exposed to Mancozeb, an EBDC formulation. In the exposed group, 40% had ETU-Hb adducts levels ranging from 0.5 to 1.42 pmol ETU/mg Hb. Such adducts might be useful for measuring EBDC exposure in humans.
| Original language | English |
|---|---|
| Pages (from-to) | 306-311 |
| Number of pages | 6 |
| Journal | Archives of Toxicology |
| Volume | 69 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Mar 1995 |
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Keywords
- Adducts
- Dosimetry
- Ethylene bisdithiocarbamate fungicides
- Ethylenethiourea
- Hemoglobin
ASJC Scopus subject areas
- Toxicology
- Health, Toxicology and Mutagenesis
Cite this
Gas chromatography-mass spectrometry determination of ethylenethiourea hemoglobin adducts : a possible indicator of exposure to ethylene bis dithiocarbamate pesticides. / Pastorelli, Roberta; Allevi, Riccardo; Romagnano, Stefano; Meli, Giovanna; Fanelli, Roberto; Airoldi, Luisa.
In: Archives of Toxicology, Vol. 69, No. 5, 03.1995, p. 306-311.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Gas chromatography-mass spectrometry determination of ethylenethiourea hemoglobin adducts
T2 - a possible indicator of exposure to ethylene bis dithiocarbamate pesticides
AU - Pastorelli, Roberta
AU - Allevi, Riccardo
AU - Romagnano, Stefano
AU - Meli, Giovanna
AU - Fanelli, Roberto
AU - Airoldi, Luisa
PY - 1995/3
Y1 - 1995/3
N2 - Ethylene bisdithiocarbamates (EBDC) are an important class of fungicides used to control crop diseases and prevent mold. Ethylenethiourea (ETU), reported to be their main degradation and metabolic product in animals and man, may have teratogenic and carcinogenic properties. The feasibility of monitoring exposure to ETU on the basis of the formation of adducts to hemoglobin (Hb) was investigated. Rats given a single oral dose of ETU (from 62.5 to 500 mg/kg body wt) formed stable covalent ETU-Hb adducts. Mild acid hydrolysis of the protein regenerated ETU, allowing its detection by isotope dilution gas chromatography-mass spectrometry (GC-MS). The amount of released ETU increased with the dose. The dose-response curve fitted a linear model only between 62.5 mg/kg and 250 mg/kg. Acid-releasable ETU was also positively identified in the hemoglobin of workers exposed to Mancozeb, an EBDC formulation. In the exposed group, 40% had ETU-Hb adducts levels ranging from 0.5 to 1.42 pmol ETU/mg Hb. Such adducts might be useful for measuring EBDC exposure in humans.
AB - Ethylene bisdithiocarbamates (EBDC) are an important class of fungicides used to control crop diseases and prevent mold. Ethylenethiourea (ETU), reported to be their main degradation and metabolic product in animals and man, may have teratogenic and carcinogenic properties. The feasibility of monitoring exposure to ETU on the basis of the formation of adducts to hemoglobin (Hb) was investigated. Rats given a single oral dose of ETU (from 62.5 to 500 mg/kg body wt) formed stable covalent ETU-Hb adducts. Mild acid hydrolysis of the protein regenerated ETU, allowing its detection by isotope dilution gas chromatography-mass spectrometry (GC-MS). The amount of released ETU increased with the dose. The dose-response curve fitted a linear model only between 62.5 mg/kg and 250 mg/kg. Acid-releasable ETU was also positively identified in the hemoglobin of workers exposed to Mancozeb, an EBDC formulation. In the exposed group, 40% had ETU-Hb adducts levels ranging from 0.5 to 1.42 pmol ETU/mg Hb. Such adducts might be useful for measuring EBDC exposure in humans.
KW - Adducts
KW - Dosimetry
KW - Ethylene bisdithiocarbamate fungicides
KW - Ethylenethiourea
KW - Hemoglobin
UR - http://www.scopus.com/inward/record.url?scp=0028985916&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028985916&partnerID=8YFLogxK
U2 - 10.1007/s002040050175
DO - 10.1007/s002040050175
M3 - Article
C2 - 7654134
AN - SCOPUS:0028985916
VL - 69
SP - 306
EP - 311
JO - Archiv fur Toxikologie
JF - Archiv fur Toxikologie
SN - 0003-9446
IS - 5
ER -