Gastric cardia carcinoma is associated with the promoter -77T>C gene polymorphism of X-Ray Cross-Complementing Group 1 (XRCC1)

Giovanni Corso, Daniele Marrelli, Corrado Pedrazzani, José Carlos Machado, Stefano Mancini, Raquel Seruca, Franco Roviello

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Purpose: X-ray repair cross complementing group 1 (XRCC1) is one of the major DNA repair proteins involved in the bas-excision repair pathway. Several single-nucleotide polymorphisms in the XRCC1 gene are identified and related with increased cancer risk development. In particular, the -77T>C polymorphism located on the promoter region relates with lung cancer risk development. The aim of this study is to analyze the -77T>C allelic frequencies in a population composed of 456 primary gastric cancer patients (GC) and 507 blood donor controls. Methods: GC patients were observed at the University of Siena, Italy; clinicopathological data and family history were available for the cancer group. The control group is composed of blood donors. Constitutional genomic DNA was PCR amplified, and XRCC1 -77T>C was detected using restriction enzyme BsrB I and analyzed in a 3% agarose gel. Results: The -77C>C homozygous genotype was significantly associated with increased risk of gastric cardia carcinoma (p = 0.023) with an odds ratio of 1. 65 (95% confidence interval 1.14 to 2.4). In the family history stratification, we report a significant association (p = 0.043) between the -77T>C polymorphism and GC cases with familial lung cancer aggregation. Conclusions: Our results suggest that the XRCC1 -77T>C polymorphism is a relevant host susceptibility factor for gastric cardia cancer development and specific subsets of familial clustering of GC.

Original languageEnglish
Pages (from-to)2233-2238
Number of pages6
JournalJournal of Gastrointestinal Surgery
Volume13
Issue number12
DOIs
Publication statusPublished - Dec 2009

Fingerprint

Cardia
Stomach Neoplasms
Stomach
X-Rays
Carcinoma
Genes
Blood Donors
DNA Repair
Lung Neoplasms
Genetic Promoter Regions
Sepharose
Italy
Single Nucleotide Polymorphism
Cluster Analysis
Neoplasms
Gels
Odds Ratio
Genotype
Confidence Intervals
Polymerase Chain Reaction

Keywords

  • Gastric cancer
  • Risk factor
  • Single nucleotide genetic polymorphism

ASJC Scopus subject areas

  • Surgery
  • Gastroenterology

Cite this

Gastric cardia carcinoma is associated with the promoter -77T>C gene polymorphism of X-Ray Cross-Complementing Group 1 (XRCC1). / Corso, Giovanni; Marrelli, Daniele; Pedrazzani, Corrado; Machado, José Carlos; Mancini, Stefano; Seruca, Raquel; Roviello, Franco.

In: Journal of Gastrointestinal Surgery, Vol. 13, No. 12, 12.2009, p. 2233-2238.

Research output: Contribution to journalArticle

Corso, Giovanni ; Marrelli, Daniele ; Pedrazzani, Corrado ; Machado, José Carlos ; Mancini, Stefano ; Seruca, Raquel ; Roviello, Franco. / Gastric cardia carcinoma is associated with the promoter -77T>C gene polymorphism of X-Ray Cross-Complementing Group 1 (XRCC1). In: Journal of Gastrointestinal Surgery. 2009 ; Vol. 13, No. 12. pp. 2233-2238.
@article{5b04ccc8d17a4771a5099bb862a6a70d,
title = "Gastric cardia carcinoma is associated with the promoter -77T>C gene polymorphism of X-Ray Cross-Complementing Group 1 (XRCC1)",
abstract = "Purpose: X-ray repair cross complementing group 1 (XRCC1) is one of the major DNA repair proteins involved in the bas-excision repair pathway. Several single-nucleotide polymorphisms in the XRCC1 gene are identified and related with increased cancer risk development. In particular, the -77T>C polymorphism located on the promoter region relates with lung cancer risk development. The aim of this study is to analyze the -77T>C allelic frequencies in a population composed of 456 primary gastric cancer patients (GC) and 507 blood donor controls. Methods: GC patients were observed at the University of Siena, Italy; clinicopathological data and family history were available for the cancer group. The control group is composed of blood donors. Constitutional genomic DNA was PCR amplified, and XRCC1 -77T>C was detected using restriction enzyme BsrB I and analyzed in a 3{\%} agarose gel. Results: The -77C>C homozygous genotype was significantly associated with increased risk of gastric cardia carcinoma (p = 0.023) with an odds ratio of 1. 65 (95{\%} confidence interval 1.14 to 2.4). In the family history stratification, we report a significant association (p = 0.043) between the -77T>C polymorphism and GC cases with familial lung cancer aggregation. Conclusions: Our results suggest that the XRCC1 -77T>C polymorphism is a relevant host susceptibility factor for gastric cardia cancer development and specific subsets of familial clustering of GC.",
keywords = "Gastric cancer, Risk factor, Single nucleotide genetic polymorphism",
author = "Giovanni Corso and Daniele Marrelli and Corrado Pedrazzani and Machado, {Jos{\'e} Carlos} and Stefano Mancini and Raquel Seruca and Franco Roviello",
year = "2009",
month = "12",
doi = "10.1007/s11605-009-0980-x",
language = "English",
volume = "13",
pages = "2233--2238",
journal = "Journal of Gastrointestinal Surgery",
issn = "1091-255X",
publisher = "Springer New York LLC",
number = "12",

}

TY - JOUR

T1 - Gastric cardia carcinoma is associated with the promoter -77T>C gene polymorphism of X-Ray Cross-Complementing Group 1 (XRCC1)

AU - Corso, Giovanni

AU - Marrelli, Daniele

AU - Pedrazzani, Corrado

AU - Machado, José Carlos

AU - Mancini, Stefano

AU - Seruca, Raquel

AU - Roviello, Franco

PY - 2009/12

Y1 - 2009/12

N2 - Purpose: X-ray repair cross complementing group 1 (XRCC1) is one of the major DNA repair proteins involved in the bas-excision repair pathway. Several single-nucleotide polymorphisms in the XRCC1 gene are identified and related with increased cancer risk development. In particular, the -77T>C polymorphism located on the promoter region relates with lung cancer risk development. The aim of this study is to analyze the -77T>C allelic frequencies in a population composed of 456 primary gastric cancer patients (GC) and 507 blood donor controls. Methods: GC patients were observed at the University of Siena, Italy; clinicopathological data and family history were available for the cancer group. The control group is composed of blood donors. Constitutional genomic DNA was PCR amplified, and XRCC1 -77T>C was detected using restriction enzyme BsrB I and analyzed in a 3% agarose gel. Results: The -77C>C homozygous genotype was significantly associated with increased risk of gastric cardia carcinoma (p = 0.023) with an odds ratio of 1. 65 (95% confidence interval 1.14 to 2.4). In the family history stratification, we report a significant association (p = 0.043) between the -77T>C polymorphism and GC cases with familial lung cancer aggregation. Conclusions: Our results suggest that the XRCC1 -77T>C polymorphism is a relevant host susceptibility factor for gastric cardia cancer development and specific subsets of familial clustering of GC.

AB - Purpose: X-ray repair cross complementing group 1 (XRCC1) is one of the major DNA repair proteins involved in the bas-excision repair pathway. Several single-nucleotide polymorphisms in the XRCC1 gene are identified and related with increased cancer risk development. In particular, the -77T>C polymorphism located on the promoter region relates with lung cancer risk development. The aim of this study is to analyze the -77T>C allelic frequencies in a population composed of 456 primary gastric cancer patients (GC) and 507 blood donor controls. Methods: GC patients were observed at the University of Siena, Italy; clinicopathological data and family history were available for the cancer group. The control group is composed of blood donors. Constitutional genomic DNA was PCR amplified, and XRCC1 -77T>C was detected using restriction enzyme BsrB I and analyzed in a 3% agarose gel. Results: The -77C>C homozygous genotype was significantly associated with increased risk of gastric cardia carcinoma (p = 0.023) with an odds ratio of 1. 65 (95% confidence interval 1.14 to 2.4). In the family history stratification, we report a significant association (p = 0.043) between the -77T>C polymorphism and GC cases with familial lung cancer aggregation. Conclusions: Our results suggest that the XRCC1 -77T>C polymorphism is a relevant host susceptibility factor for gastric cardia cancer development and specific subsets of familial clustering of GC.

KW - Gastric cancer

KW - Risk factor

KW - Single nucleotide genetic polymorphism

UR - http://www.scopus.com/inward/record.url?scp=71149103691&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=71149103691&partnerID=8YFLogxK

U2 - 10.1007/s11605-009-0980-x

DO - 10.1007/s11605-009-0980-x

M3 - Article

VL - 13

SP - 2233

EP - 2238

JO - Journal of Gastrointestinal Surgery

JF - Journal of Gastrointestinal Surgery

SN - 1091-255X

IS - 12

ER -