TY - JOUR
T1 - Gastric mucosa as an additional extrahepatic localization of hepatitis C virus
T2 - Viral detection in gastric low-grade lymphoma associated with autoimmune disease and in chronic gastritis
AU - De Vita, Salvatore
AU - De Valli, R. E.
AU - Sansonno, Domenico
AU - Sorrentino, Dario
AU - La Corte, Renato
AU - Pivetta, Barbara
AU - Gasparotto, Daniela
AU - Racanelli, Vito
AU - Marzotto, Alessandra
AU - Labombarda, Antonella
AU - Gloghini, Annunziata
AU - Ferraccioli, Glanfranco
AU - Monteverde, Angelo
AU - Carbone, Antonino
AU - Dammacco, Franco
AU - Boiocchi, Mauro
PY - 2000
Y1 - 2000
N2 - The hepatitis C virus (HCV) has been linked to B-cell lymphoproliferation and autoimmunity, and has been localized in several tissues. The clinical observation of an HCV-infected patient with Sjogren's syndrome (SS) and Helicobacter pylori (HP) positive gastric low-grade B-cell non-Hodgkin's lymphoma (NHL), which did not regress after HP eradication, led us to investigate the possible localization of HVC in the gastric microenvironment. HCV genome and antigens were searched in gastric biopsy specimens from the previously mentioned case, as well as from 9 additional HCV-infected patients (8 with chronic gastritis and 1 with gastric low-grade B-cell NHL). HCV-specific polymerase chain reaction (PCR) and immunohistochemistry procedures were used. The gastric B-cell NHL from the patient with SS was characterized by molecular analyses of B-cell clonality. HCV RNA was detected in both the gastric low-grade B-cell NHL and in 3 out of 6 gastric samples from the remaining cases. HCV antigens were detected in the residual glandular cells within the gastric B-cell NHL lesions, in glandular cells from 2 of the 3 additional gastric lesions that were HCV positive by PCR, and in 1 additional chronic gastritis sample in which HCV-RNA studies could not be performed. By molecular analyses, of immunoglobulin genes, the B-cell NHL from the patient with SS was confirmed to be a primary gastrilymphoma, subjected to ongoing antigenic stimulation and showing a significant similarity with rheumatoid factor (RF) and anti-HCV-antibody sequences. Our results show that HCV can localize in the gastric mucosa.
AB - The hepatitis C virus (HCV) has been linked to B-cell lymphoproliferation and autoimmunity, and has been localized in several tissues. The clinical observation of an HCV-infected patient with Sjogren's syndrome (SS) and Helicobacter pylori (HP) positive gastric low-grade B-cell non-Hodgkin's lymphoma (NHL), which did not regress after HP eradication, led us to investigate the possible localization of HVC in the gastric microenvironment. HCV genome and antigens were searched in gastric biopsy specimens from the previously mentioned case, as well as from 9 additional HCV-infected patients (8 with chronic gastritis and 1 with gastric low-grade B-cell NHL). HCV-specific polymerase chain reaction (PCR) and immunohistochemistry procedures were used. The gastric B-cell NHL from the patient with SS was characterized by molecular analyses of B-cell clonality. HCV RNA was detected in both the gastric low-grade B-cell NHL and in 3 out of 6 gastric samples from the remaining cases. HCV antigens were detected in the residual glandular cells within the gastric B-cell NHL lesions, in glandular cells from 2 of the 3 additional gastric lesions that were HCV positive by PCR, and in 1 additional chronic gastritis sample in which HCV-RNA studies could not be performed. By molecular analyses, of immunoglobulin genes, the B-cell NHL from the patient with SS was confirmed to be a primary gastrilymphoma, subjected to ongoing antigenic stimulation and showing a significant similarity with rheumatoid factor (RF) and anti-HCV-antibody sequences. Our results show that HCV can localize in the gastric mucosa.
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M3 - Article
C2 - 10613744
AN - SCOPUS:0005708373
VL - 31
SP - 182
EP - 189
JO - Hepatology
JF - Hepatology
SN - 0270-9139
IS - 1
ER -