TY - JOUR
T1 - Gathering novel circulating exosomal microRNA in osteosarcoma cell lines and possible implications for the disease
AU - Cuscino, Nicola
AU - Raimondi, Lavinia
AU - De Luca, Angela
AU - Carcione, Claudia
AU - Russelli, Giovanna
AU - Conti, Laura
AU - Baldi, Jacopo
AU - Giulio Conaldi, Pier
AU - Giavaresi, Gianluca
AU - Gallo, Alessia
PY - 2019/12
Y1 - 2019/12
N2 - One of the goals of personalized medicine is to understand and treat diseases with greater precision through the molecular profile of the patient. This profiling is becoming a powerful tool for the discovery of novel biomarkers that can guide physicians in assessing, in advance, the disease stage, and monitoring disease progression. Circulating miRNAs and exosomal miRNAs, a group of small non-coding RNAs, are considered the gold standard diagnostic biomarkers for human diseases. We have previously demonstrated that osteosarcoma-derived exosomes are able to influence crucial mechanisms inside tumor niches, inducing osteoclast differentiation, and sustaining bone resorption activity. Here we discovered, through Next-Generation Sequencing (NGS), eight novel microRNAs in three different osteosarcoma cell lines, and assessed the selective packaging into the exosomes released. We then investigated, as proof-of-principle, the presence of the novel microRNAs in osteosarcoma patient samples, and found that 5 of the 8 novel microRNAs were more present in circulating exosomes of osteosarcoma patients compared with the controls. These results raise a question: Could the 8 novel microRNAs play a role for osteosarcoma pathogenesis? Although still premature, the results are encouraging, and further studies with a validation in a larger cohort are needed.
AB - One of the goals of personalized medicine is to understand and treat diseases with greater precision through the molecular profile of the patient. This profiling is becoming a powerful tool for the discovery of novel biomarkers that can guide physicians in assessing, in advance, the disease stage, and monitoring disease progression. Circulating miRNAs and exosomal miRNAs, a group of small non-coding RNAs, are considered the gold standard diagnostic biomarkers for human diseases. We have previously demonstrated that osteosarcoma-derived exosomes are able to influence crucial mechanisms inside tumor niches, inducing osteoclast differentiation, and sustaining bone resorption activity. Here we discovered, through Next-Generation Sequencing (NGS), eight novel microRNAs in three different osteosarcoma cell lines, and assessed the selective packaging into the exosomes released. We then investigated, as proof-of-principle, the presence of the novel microRNAs in osteosarcoma patient samples, and found that 5 of the 8 novel microRNAs were more present in circulating exosomes of osteosarcoma patients compared with the controls. These results raise a question: Could the 8 novel microRNAs play a role for osteosarcoma pathogenesis? Although still premature, the results are encouraging, and further studies with a validation in a larger cohort are needed.
KW - Exosomes
KW - Liquid biopsy This research received no external funding
KW - MicroRNAs
UR - http://www.scopus.com/inward/record.url?scp=85076164089&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85076164089&partnerID=8YFLogxK
U2 - 10.3390/cancers11121924
DO - 10.3390/cancers11121924
M3 - Article
AN - SCOPUS:85076164089
VL - 11
JO - Cancers
JF - Cancers
SN - 2072-6694
IS - 12
M1 - 1924
ER -