GD3 glycosphingolipid contributes to Fas-mediated apoptosis via association with ezrin cytoskeletal protein

Anna Maria Giammarioli, Tina Garofalo, Maurizio Sorice, Roberta Misasi, Lucrezia Gambardella, Roberto Gradini, Stefano Fais, Antonio Pavan, Walter Malorni

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Efficiency of Fas-mediated apoptosis of lymphoid cells is regulated, among other means, by a mechanism involving its association with ezrin, a cytoskeletal protein belonging to the 4.1 family of proteins. In the present work, we provide evidence for a further molecule that associates to ezrin in Fas-triggered apoptosis, the disialoganglioside GD3. In fact, as an early event, GD3 redistributed in membrane-associated domains in uropods and co-localized with ezrin. Co-immunoprecipitation analyses confirmed this result, indicating a GD3-ezrin association. Altogether, these results are suggestive for a role of GD3 in Fas/ezrin-mediated apoptosis, supporting the view that uropods contain a multimolecular signaling complex involved in Fas-mediated apoptosis.

Original languageEnglish
Pages (from-to)45-50
Number of pages6
JournalFEBS Letters
Volume506
Issue number1
DOIs
Publication statusPublished - Sep 28 2001

Fingerprint

Glycosphingolipids
Cytoskeletal Proteins
Apoptosis
Immunoprecipitation
ezrin
Lymphocytes
Membranes
Molecules
Proteins

Keywords

  • Apoptosis
  • Ezrin
  • Fas
  • Ganglioside
  • GD3

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Giammarioli, A. M., Garofalo, T., Sorice, M., Misasi, R., Gambardella, L., Gradini, R., ... Malorni, W. (2001). GD3 glycosphingolipid contributes to Fas-mediated apoptosis via association with ezrin cytoskeletal protein. FEBS Letters, 506(1), 45-50. https://doi.org/10.1016/S0014-5793(01)02776-4

GD3 glycosphingolipid contributes to Fas-mediated apoptosis via association with ezrin cytoskeletal protein. / Giammarioli, Anna Maria; Garofalo, Tina; Sorice, Maurizio; Misasi, Roberta; Gambardella, Lucrezia; Gradini, Roberto; Fais, Stefano; Pavan, Antonio; Malorni, Walter.

In: FEBS Letters, Vol. 506, No. 1, 28.09.2001, p. 45-50.

Research output: Contribution to journalArticle

Giammarioli, AM, Garofalo, T, Sorice, M, Misasi, R, Gambardella, L, Gradini, R, Fais, S, Pavan, A & Malorni, W 2001, 'GD3 glycosphingolipid contributes to Fas-mediated apoptosis via association with ezrin cytoskeletal protein', FEBS Letters, vol. 506, no. 1, pp. 45-50. https://doi.org/10.1016/S0014-5793(01)02776-4
Giammarioli AM, Garofalo T, Sorice M, Misasi R, Gambardella L, Gradini R et al. GD3 glycosphingolipid contributes to Fas-mediated apoptosis via association with ezrin cytoskeletal protein. FEBS Letters. 2001 Sep 28;506(1):45-50. https://doi.org/10.1016/S0014-5793(01)02776-4
Giammarioli, Anna Maria ; Garofalo, Tina ; Sorice, Maurizio ; Misasi, Roberta ; Gambardella, Lucrezia ; Gradini, Roberto ; Fais, Stefano ; Pavan, Antonio ; Malorni, Walter. / GD3 glycosphingolipid contributes to Fas-mediated apoptosis via association with ezrin cytoskeletal protein. In: FEBS Letters. 2001 ; Vol. 506, No. 1. pp. 45-50.
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