Gelsolin secretion in interleukin-4-treated bronchial epithelia and in asthmatic airways

Giovanni Candiano, Maurizio Bruschi, Nicoletta Pedemonte, Emanuela Caci, Sabrina Liberatori, Luca Bini, Carlo Pellegrini, Mario Viganò, Brian J. O'Connor, Tak H. Lee, Luis J V Galietta, Olga Zegarra-Moran

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Rationale: The airway surface liquid, the thin layer of liquid covering the airways, is essential for mucociliary clearance and as a barrier against microbial and other noxious agents. Proteins secreted into the airway surface liquid by epithelial and nonepithelial cells may be important in innate immunity and to improve the fluidity of mucous secretions. Objectives: We aimed to identify proteins specifically secreted into the airway surface liquid by human bronchial epithelial cells, in resting conditions and after treatment with interleukin 4 (IL-4), a cytokine released in asthma. Methods and Main Results: By using a proteomics approach, we found that one of the most abundant proteins was gelsolin, which breaks down actin filaments. Gelsolin mRNA and protein secretion were increased threefold in the airway surface liquid of epithelia treated with IL-4. These results were confirmed at the functional level by measuring actin depolymerization using a fluorescence assay. Gelsolin protein was also upregulated in the airways of subjects with asthma. Conclusions: Our findings indicate that gelsolin is released by epithelial cells into the airways and that its secretion is increased by IL-4 in vitro. In addition, we found that the concentration of both IL-4 and gelsolin were raised in the bronchoalveolar lavage of patients with asthma. These results suggest that gelsolin might improve the fluidity of airway surface liquid in asthma by breaking down filamentous actin that may be released in large amounts by dying cells during inflammation.

Original languageEnglish
Pages (from-to)1090-1096
Number of pages7
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume172
Issue number9
DOIs
Publication statusPublished - Nov 1 2005

Fingerprint

Gelsolin
Interleukin-4
Epithelium
Asthma
Epithelial Cells
Actins
Mucociliary Clearance
Bronchoalveolar Lavage
Actin Cytoskeleton
Innate Immunity
Proteomics
Proteins
Fluorescence
Cytokines
Inflammation
Messenger RNA

Keywords

  • Actin
  • Epithelium
  • Mucociliary clearance

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Gelsolin secretion in interleukin-4-treated bronchial epithelia and in asthmatic airways. / Candiano, Giovanni; Bruschi, Maurizio; Pedemonte, Nicoletta; Caci, Emanuela; Liberatori, Sabrina; Bini, Luca; Pellegrini, Carlo; Viganò, Mario; O'Connor, Brian J.; Lee, Tak H.; Galietta, Luis J V; Zegarra-Moran, Olga.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 172, No. 9, 01.11.2005, p. 1090-1096.

Research output: Contribution to journalArticle

Candiano, Giovanni ; Bruschi, Maurizio ; Pedemonte, Nicoletta ; Caci, Emanuela ; Liberatori, Sabrina ; Bini, Luca ; Pellegrini, Carlo ; Viganò, Mario ; O'Connor, Brian J. ; Lee, Tak H. ; Galietta, Luis J V ; Zegarra-Moran, Olga. / Gelsolin secretion in interleukin-4-treated bronchial epithelia and in asthmatic airways. In: American Journal of Respiratory and Critical Care Medicine. 2005 ; Vol. 172, No. 9. pp. 1090-1096.
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