TY - JOUR
T1 - Gene and protein expression in response to different growth temperatures and oxygen availability in Burkholderia thailandensis
AU - Peano, Clelia
AU - Chiaramonte, Fabrizio
AU - Motta, Sara
AU - Pietrelli, Alessandro
AU - Jaillon, Sebastien
AU - Rossi, Elio
AU - Consolandi, Clarissa
AU - Champion, Olivia L.
AU - Michell, Stephen L.
AU - Freddi, Luca
AU - Falciola, Luigi
AU - Basilico, Fabrizio
AU - Garlanda, Cecilia
AU - Mauri, Pierluigi
AU - De Bellis, Gianluca
AU - Landini, Paolo
PY - 2014/3/26
Y1 - 2014/3/26
N2 - Burkholderia thailandensis, although normally avirulent for mammals, can infect macrophages in vitro and has occasionally been reported to cause pneumonia in humans. It is therefore used as a model organism for the human pathogen B. pseudomallei, to which it is closely related phylogenetically. We characterized the B. thailandensis clinical isolate CDC2721121 (BtCDC272) at the genome level and studied its response to environmental cues associated with human host colonization, namely, temperature and oxygen limitation. Effects of the different growth conditions on BtCDC272 were studied through whole genome transcription studies and analysis of proteins associated with the bacterial cell surface. We found that growth at 37°C, compared to 28°C, negatively affected cell motility and flagella production through a mechanism involving regulation of the flagellin-encoding fliC gene at the mRNA stability level. Growth in oxygen-limiting conditions, in contrast, stimulated various processes linked to virulence, such as lipopolysaccharide production and expression of genes encoding protein secretion systems. Consistent with these observations, BtCDC272 grown in oxygen limitation was more resistant to phagocytosis and strongly induced the production of inflammatory cytokines from murine macrophages. Our results suggest that, while temperature sensing is important for regulation of B. thailandensis cell motility, oxygen limitation has a deeper impact on its physiology and constitutes a crucial environmental signal for the production of virulence factors.
AB - Burkholderia thailandensis, although normally avirulent for mammals, can infect macrophages in vitro and has occasionally been reported to cause pneumonia in humans. It is therefore used as a model organism for the human pathogen B. pseudomallei, to which it is closely related phylogenetically. We characterized the B. thailandensis clinical isolate CDC2721121 (BtCDC272) at the genome level and studied its response to environmental cues associated with human host colonization, namely, temperature and oxygen limitation. Effects of the different growth conditions on BtCDC272 were studied through whole genome transcription studies and analysis of proteins associated with the bacterial cell surface. We found that growth at 37°C, compared to 28°C, negatively affected cell motility and flagella production through a mechanism involving regulation of the flagellin-encoding fliC gene at the mRNA stability level. Growth in oxygen-limiting conditions, in contrast, stimulated various processes linked to virulence, such as lipopolysaccharide production and expression of genes encoding protein secretion systems. Consistent with these observations, BtCDC272 grown in oxygen limitation was more resistant to phagocytosis and strongly induced the production of inflammatory cytokines from murine macrophages. Our results suggest that, while temperature sensing is important for regulation of B. thailandensis cell motility, oxygen limitation has a deeper impact on its physiology and constitutes a crucial environmental signal for the production of virulence factors.
UR - http://www.scopus.com/inward/record.url?scp=84899847958&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84899847958&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0093009
DO - 10.1371/journal.pone.0093009
M3 - Article
C2 - 24671187
AN - SCOPUS:84899847958
VL - 9
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 3
M1 - e93009
ER -