Gene and protein expression of CXCR4 in adult and elderly patients with chronic rhinitis, pharyngitis or sinusitis undergoing thermal water nasal inhalations

Monica Neri, Luigi Sansone, Luisa Pietrasanta, Aliaksei Kisialiou, Eloisa Cabano, Marina Martini, Matteo A. Russo, Donatella Ugolini, Marco Tafani, Stefano Bonassi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Chronic rhinitis, pharyngitis and sinusitis are common health problems with a significant impact on public health, and are suspected to be influenced by ageing factors. Nasal inhalation with thermal water may be used to reduce symptoms, inflammation and drug intake. A pre-post clinical study was conducted in 183 consecutive adult and elderly patients with chronic rhinitis, pharyngitis or sinusitis, to evaluate whether thermal water nasal inhalations could improve their symptoms, clinical signs and rhinomanometry measurements, and influence inflammatory biomarkers levels in nasal epithelial cells. Results: Participants profile revealed that they were aged on average (mean age and SD 60.6±15.2years, median 65, range 20-86, 86 aged ≤65years (47%), 96 aged >65years (53%)) and extremely concerned about wellbeing. Older age was associated with better compliance to inhalation treatment. Total symptom and clinical evaluation scores were significantly ameliorated after treatment (p<0.001), with no substantial difference according to age, while rhinomanometry results were inconsistent. Persistence of symptom improvement was confirmed at phone follow up 1 year later (n=74). The training set of 48 inflammatory genes (40 patients) revealed a strong increase of CXCR4 gene expression after nasal inhalations, confirmed both in the validation set (143 patients; 1.2±0.68 vs 3.3±1.2; p<0.0001) and by evaluation of CXCR4 protein expression (40 patients; 1.0±0.39 vs 2.6±0.66; p<0.0001). CXCR4 expression was consistently changed in patients with rhinitis, pharyngitis or sinusitis. The increase was smaller in current smokers compared to non-smokers. Results were substantially unchanged when comparing aged subjects (≥65years) or the eldest quartile (≥71years) to the others. Other genes showed weaker variations (e.g. FLT1 was reduced only in patients with sinusitis). Conclusions: These results confirm the clinical impact of thermal water nasal inhalations on upper respiratory diseases both in adults and elders, and emphasize the role of genes activating tissue repair and inflammatory pathways. Future studies should evaluate CXCR4 as possible therapeutic target or response predictor in patients with chronic rhinitis, pharyngitis or sinusitis.

Original languageEnglish
Article number10
JournalImmunity and Ageing
Volume15
Issue number1
DOIs
Publication statusPublished - Feb 27 2018

Fingerprint

Pharyngitis
Sinusitis
Rhinitis
Nose
Inhalation
Hot Temperature
Gene Expression
Water
Rhinomanometry
Proteins
Genes
Symptom Assessment
Signs and Symptoms
Therapeutics
Public Health
Biomarkers
Epithelial Cells
Inflammation
Health
Pharmaceutical Preparations

Keywords

  • Balneology
  • Clinical trial
  • CXCR4
  • Gene expression profiling
  • Inflammation
  • Respiratory tract infections

ASJC Scopus subject areas

  • Immunology
  • Ageing

Cite this

Gene and protein expression of CXCR4 in adult and elderly patients with chronic rhinitis, pharyngitis or sinusitis undergoing thermal water nasal inhalations. / Neri, Monica; Sansone, Luigi; Pietrasanta, Luisa; Kisialiou, Aliaksei; Cabano, Eloisa; Martini, Marina; Russo, Matteo A.; Ugolini, Donatella; Tafani, Marco; Bonassi, Stefano.

In: Immunity and Ageing, Vol. 15, No. 1, 10, 27.02.2018.

Research output: Contribution to journalArticle

@article{05aa7f12e5384e779fe25e7168a8665c,
title = "Gene and protein expression of CXCR4 in adult and elderly patients with chronic rhinitis, pharyngitis or sinusitis undergoing thermal water nasal inhalations",
abstract = "Background: Chronic rhinitis, pharyngitis and sinusitis are common health problems with a significant impact on public health, and are suspected to be influenced by ageing factors. Nasal inhalation with thermal water may be used to reduce symptoms, inflammation and drug intake. A pre-post clinical study was conducted in 183 consecutive adult and elderly patients with chronic rhinitis, pharyngitis or sinusitis, to evaluate whether thermal water nasal inhalations could improve their symptoms, clinical signs and rhinomanometry measurements, and influence inflammatory biomarkers levels in nasal epithelial cells. Results: Participants profile revealed that they were aged on average (mean age and SD 60.6±15.2years, median 65, range 20-86, 86 aged ≤65years (47{\%}), 96 aged >65years (53{\%})) and extremely concerned about wellbeing. Older age was associated with better compliance to inhalation treatment. Total symptom and clinical evaluation scores were significantly ameliorated after treatment (p<0.001), with no substantial difference according to age, while rhinomanometry results were inconsistent. Persistence of symptom improvement was confirmed at phone follow up 1 year later (n=74). The training set of 48 inflammatory genes (40 patients) revealed a strong increase of CXCR4 gene expression after nasal inhalations, confirmed both in the validation set (143 patients; 1.2±0.68 vs 3.3±1.2; p<0.0001) and by evaluation of CXCR4 protein expression (40 patients; 1.0±0.39 vs 2.6±0.66; p<0.0001). CXCR4 expression was consistently changed in patients with rhinitis, pharyngitis or sinusitis. The increase was smaller in current smokers compared to non-smokers. Results were substantially unchanged when comparing aged subjects (≥65years) or the eldest quartile (≥71years) to the others. Other genes showed weaker variations (e.g. FLT1 was reduced only in patients with sinusitis). Conclusions: These results confirm the clinical impact of thermal water nasal inhalations on upper respiratory diseases both in adults and elders, and emphasize the role of genes activating tissue repair and inflammatory pathways. Future studies should evaluate CXCR4 as possible therapeutic target or response predictor in patients with chronic rhinitis, pharyngitis or sinusitis.",
keywords = "Balneology, Clinical trial, CXCR4, Gene expression profiling, Inflammation, Respiratory tract infections",
author = "Monica Neri and Luigi Sansone and Luisa Pietrasanta and Aliaksei Kisialiou and Eloisa Cabano and Marina Martini and Russo, {Matteo A.} and Donatella Ugolini and Marco Tafani and Stefano Bonassi",
year = "2018",
month = "2",
day = "27",
doi = "10.1186/s12979-018-0114-y",
language = "English",
volume = "15",
journal = "Immunity and Ageing",
issn = "1742-4933",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Gene and protein expression of CXCR4 in adult and elderly patients with chronic rhinitis, pharyngitis or sinusitis undergoing thermal water nasal inhalations

AU - Neri, Monica

AU - Sansone, Luigi

AU - Pietrasanta, Luisa

AU - Kisialiou, Aliaksei

AU - Cabano, Eloisa

AU - Martini, Marina

AU - Russo, Matteo A.

AU - Ugolini, Donatella

AU - Tafani, Marco

AU - Bonassi, Stefano

PY - 2018/2/27

Y1 - 2018/2/27

N2 - Background: Chronic rhinitis, pharyngitis and sinusitis are common health problems with a significant impact on public health, and are suspected to be influenced by ageing factors. Nasal inhalation with thermal water may be used to reduce symptoms, inflammation and drug intake. A pre-post clinical study was conducted in 183 consecutive adult and elderly patients with chronic rhinitis, pharyngitis or sinusitis, to evaluate whether thermal water nasal inhalations could improve their symptoms, clinical signs and rhinomanometry measurements, and influence inflammatory biomarkers levels in nasal epithelial cells. Results: Participants profile revealed that they were aged on average (mean age and SD 60.6±15.2years, median 65, range 20-86, 86 aged ≤65years (47%), 96 aged >65years (53%)) and extremely concerned about wellbeing. Older age was associated with better compliance to inhalation treatment. Total symptom and clinical evaluation scores were significantly ameliorated after treatment (p<0.001), with no substantial difference according to age, while rhinomanometry results were inconsistent. Persistence of symptom improvement was confirmed at phone follow up 1 year later (n=74). The training set of 48 inflammatory genes (40 patients) revealed a strong increase of CXCR4 gene expression after nasal inhalations, confirmed both in the validation set (143 patients; 1.2±0.68 vs 3.3±1.2; p<0.0001) and by evaluation of CXCR4 protein expression (40 patients; 1.0±0.39 vs 2.6±0.66; p<0.0001). CXCR4 expression was consistently changed in patients with rhinitis, pharyngitis or sinusitis. The increase was smaller in current smokers compared to non-smokers. Results were substantially unchanged when comparing aged subjects (≥65years) or the eldest quartile (≥71years) to the others. Other genes showed weaker variations (e.g. FLT1 was reduced only in patients with sinusitis). Conclusions: These results confirm the clinical impact of thermal water nasal inhalations on upper respiratory diseases both in adults and elders, and emphasize the role of genes activating tissue repair and inflammatory pathways. Future studies should evaluate CXCR4 as possible therapeutic target or response predictor in patients with chronic rhinitis, pharyngitis or sinusitis.

AB - Background: Chronic rhinitis, pharyngitis and sinusitis are common health problems with a significant impact on public health, and are suspected to be influenced by ageing factors. Nasal inhalation with thermal water may be used to reduce symptoms, inflammation and drug intake. A pre-post clinical study was conducted in 183 consecutive adult and elderly patients with chronic rhinitis, pharyngitis or sinusitis, to evaluate whether thermal water nasal inhalations could improve their symptoms, clinical signs and rhinomanometry measurements, and influence inflammatory biomarkers levels in nasal epithelial cells. Results: Participants profile revealed that they were aged on average (mean age and SD 60.6±15.2years, median 65, range 20-86, 86 aged ≤65years (47%), 96 aged >65years (53%)) and extremely concerned about wellbeing. Older age was associated with better compliance to inhalation treatment. Total symptom and clinical evaluation scores were significantly ameliorated after treatment (p<0.001), with no substantial difference according to age, while rhinomanometry results were inconsistent. Persistence of symptom improvement was confirmed at phone follow up 1 year later (n=74). The training set of 48 inflammatory genes (40 patients) revealed a strong increase of CXCR4 gene expression after nasal inhalations, confirmed both in the validation set (143 patients; 1.2±0.68 vs 3.3±1.2; p<0.0001) and by evaluation of CXCR4 protein expression (40 patients; 1.0±0.39 vs 2.6±0.66; p<0.0001). CXCR4 expression was consistently changed in patients with rhinitis, pharyngitis or sinusitis. The increase was smaller in current smokers compared to non-smokers. Results were substantially unchanged when comparing aged subjects (≥65years) or the eldest quartile (≥71years) to the others. Other genes showed weaker variations (e.g. FLT1 was reduced only in patients with sinusitis). Conclusions: These results confirm the clinical impact of thermal water nasal inhalations on upper respiratory diseases both in adults and elders, and emphasize the role of genes activating tissue repair and inflammatory pathways. Future studies should evaluate CXCR4 as possible therapeutic target or response predictor in patients with chronic rhinitis, pharyngitis or sinusitis.

KW - Balneology

KW - Clinical trial

KW - CXCR4

KW - Gene expression profiling

KW - Inflammation

KW - Respiratory tract infections

UR - http://www.scopus.com/inward/record.url?scp=85042556050&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85042556050&partnerID=8YFLogxK

U2 - 10.1186/s12979-018-0114-y

DO - 10.1186/s12979-018-0114-y

M3 - Article

AN - SCOPUS:85042556050

VL - 15

JO - Immunity and Ageing

JF - Immunity and Ageing

SN - 1742-4933

IS - 1

M1 - 10

ER -