Gene delivery targeted to oligodendrocytes using a lentiviral vector

Alexia Kagiava, Irene Sargiannidou, Stavros Bashiardes, Jan Richter, Natasa Schiza, Christina Christodoulou, Angela Gritti, Kleopas A. Kleopa

Research output: Contribution to journalArticle

Abstract

Background: Most leukodystrophies result from mutations in genes expressed in oligodendrocytes that may cause autonomous loss of function of cell structural proteins. Therefore, effective gene delivery to oligodendrocytes is necessary to develop future treatments. Materials: To achieve this, we cloned a lentiviral vector in which the enhanced green fluorescent protein (EGFP) expression was driven by the oligodendrocyte specific 2,3-cyclic nucleotide 3-phosphodiesterase promoter. The vector was inserted into C57BL/6 neonatal mouse brain by combined intraventricular and parenchymal injections. Results: Assessment of EGFP expression revealed a widespread distribution, specifically in cells of the oligodendrocyte linage, starting from postnatal day 6 (P6) in the subventricular zone and spreading through migrating oligodendrocyte precursors. By P30, it was detectable throughout the brain and persisted for at least 3 months, showing an increase both in the number of expressing cells and in intensity over time. EGFP expression was restricted to oligodendrocyte linage cells. On average, 20.3 ±2.56% of all oligodendrocytes in different central nervous system areas were EGFP-positive, with regional variations. Conclusions: Lentiviral gene delivery using an oligodendrocyte-specific promoter may achieve widespread and long-lasting expression selectively in oligodendrocytes, offering a possibility for gene therapy in certain leukodystrophies, although the relatively low rates of oligodendrocyte transduction are a limitation that remains to be overcome.

Original languageEnglish
Pages (from-to)364-373
Number of pages10
JournalJournal of Gene Medicine
Volume16
Issue number11-12
DOIs
Publication statusPublished - Nov 1 2014

Keywords

  • CNP promoter
  • EGFP
  • Gene therapy
  • Leukodystrophy

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Molecular Medicine
  • Genetics(clinical)
  • Drug Discovery
  • Medicine(all)

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  • Cite this

    Kagiava, A., Sargiannidou, I., Bashiardes, S., Richter, J., Schiza, N., Christodoulou, C., Gritti, A., & Kleopa, K. A. (2014). Gene delivery targeted to oligodendrocytes using a lentiviral vector. Journal of Gene Medicine, 16(11-12), 364-373. https://doi.org/10.1002/jgm.2813