Gene dosage in capillary electrophoresis

Pre-natal diagnosis of Down's syndrome

Cecilia Gelfi, Gianfranco Cossu, Piera Carta, Maddalena Serra, Pier Giorgio Righetti

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Modern proposals for pre-natal genetic analysis of Down's syndrome consist in isolating DNA from amniotic cells and amplifying a highly polymorphic small tandem repeat region of the chromosome 21-specific D21S11 marker. The polymerase-chain-reaction-amplified fragments are typically 5′-end labelled with a green or blue fluorescent reporter and data acquisition occurs on-lane in DNA sequencing gel-slabs and equipment. The following patterns are expected: for normal individuals, 1 peak or two peaks in a 1:1 ratio. In the case of trisomy 21, the following patterns are found: either three peaks in a 1:1:1 ratio or a two-peak profile with a 2:1 gene ratio. We have developed a capillary electrophoretic system, offering precise diagnostic value by exploiting the intrinsic DNA absorbance at 254 nm. The separation occurs in capillaries coated with an extremely stable and hydrophilic layer of poly(N-acroyloyl amino ethoxy ethanol) and filled with a background electrolyte consisting of 89 mM Tris-borate, 2 mM EDTA, 2.5 μM ethidium bromide and 8% short-chain, low-viscosity, replaceable, liquid, linear, sieving polyacrylamide. The technique offers high reproducibility and precise on-liine, automated peak acquisition and quantitation.

Original languageEnglish
Pages (from-to)405-412
Number of pages8
JournalJournal of Chromatography A
Volume718
Issue number2
DOIs
Publication statusPublished - Dec 22 1995

Fingerprint

Capillary electrophoresis
Gene Dosage
Capillary Electrophoresis
Down Syndrome
Genes
Chromosomes, Human, Pair 21
Borates
Tandem Repeat Sequences
Ethidium
DNA
DNA Sequence Analysis
Viscosity
Edetic Acid
Electrolytes
Ethanol
Gels
Polymerase chain reaction
Chromosomes
Equipment and Supplies
Polymerase Chain Reaction

Keywords

  • Capillary electrophoresis
  • DNA
  • Down's syndrome
  • Gene dosage

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Gene dosage in capillary electrophoresis : Pre-natal diagnosis of Down's syndrome. / Gelfi, Cecilia; Cossu, Gianfranco; Carta, Piera; Serra, Maddalena; Righetti, Pier Giorgio.

In: Journal of Chromatography A, Vol. 718, No. 2, 22.12.1995, p. 405-412.

Research output: Contribution to journalArticle

Gelfi, Cecilia ; Cossu, Gianfranco ; Carta, Piera ; Serra, Maddalena ; Righetti, Pier Giorgio. / Gene dosage in capillary electrophoresis : Pre-natal diagnosis of Down's syndrome. In: Journal of Chromatography A. 1995 ; Vol. 718, No. 2. pp. 405-412.
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