Gene dosage mutants at adenine phosphoribosyltransferase locus induced by colcemid in Chinese hamster V79-AP4 cells

Carlo M. Colella, Silvana Simi, Giuseppe Rainaldi, Theo van Boxel, Giuliano Della Valle, Antonio F. Peverali, Daniela Talarico, Tullio Mariani, Antonio Piras, Marcella Simili

Research output: Contribution to journalArticle

Abstract

Pseudodiploid Chinese hamster V79-AP4 cells, functionally diploid at the adenine phosphoribosyltransferase (aprt)locus, were treated with colcemid, a well-known aneuploidizing agent, under various experimental conditions. Aneuploid and tetraploid cells and variants resistant to 10 μg/ml of 2,6-diaminopurine (DAP), which selects for presumptive aprt+/-heterozygotes in the untreated cells, were induced. Many of the induced variants were hypotetraploid with three (rather than four) chromosomes carrying the aprt gene. Dot-blot and Southern analysis of the DNA of these clones confirmed that they had three copies of the aprt gene. Their APRT specific enzymatic activity was 60-80% of that of wild-type V79-AP4. The results of these and other experiments suggest that in these variants resistance to DAP is due to an altered aprt gene dosage and point to a possible genetic effect of colcemid and other aneuploidizing agents in somatic mammalian cells.

Original languageEnglish
Pages (from-to)593-604
Number of pages12
JournalSomatic Cell and Molecular Genetics
Volume14
Issue number6
DOIs
Publication statusPublished - Nov 1988

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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    Colella, C. M., Simi, S., Rainaldi, G., van Boxel, T., Della Valle, G., Peverali, A. F., Talarico, D., Mariani, T., Piras, A., & Simili, M. (1988). Gene dosage mutants at adenine phosphoribosyltransferase locus induced by colcemid in Chinese hamster V79-AP4 cells. Somatic Cell and Molecular Genetics, 14(6), 593-604. https://doi.org/10.1007/BF01535313