Abstract
Background: Pancreatic cancer is one of the leading causes of cancer-related death. Using DNA gene expression analysis based on a custom made Affymetrix cancer array, we investigated the expression pattern of both primary and established pancreatic carcinoma cell lines. Methods: We analyzed the gene expression of 5 established pancreatic cancer cell lines (AsPC-1, BxPC-3, Capan-1, Capan-2 and HPAF II) and 5 primary isolates, 1 of them derived from benign pancreatic duct cells. Results: Out of 1,540 genes which were expressed in at least 3 experiments, we found 122 genes upregulated and 18 downregulated in tumor cell lines compared to benign cells with a fold change >3. Several of the upregulated genes (like Prefoldin 5, ADAM9 and E-cadherin) have been associated with pancreatic cancer before. The other differentially regulated genes, however, play a so far unknown role in the course of human pancreatic carcinoma. By means of immunohistochemistry we could show that thymosin β-10 (TMSB10), upregulated in tumor cell lines, is expressed in human pancreatic carcinoma, but not in non-neoplastic pancreatictissue, suggesting a role for TMSB10 in the carcinogenesis of pancreatic carcinoma. Conclusion: Using gene expression profiling of pancreatic cell lines we were able to identify genes differentially expressed in pancreatic adenocarcinoma, which might contribute to pancreatic cancer development.
| Original language | English |
|---|---|
| Pages (from-to) | 370-379 |
| Number of pages | 10 |
| Journal | Pancreatology |
| Volume | 5 |
| Issue number | 4-5 |
| DOIs | |
| Publication status | Published - 2005 |
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Keywords
- Cancer
- Cell line
- Immunohistochemistry
- Microarray
- Pancreas
- Primary isolates
- Thymosin
ASJC Scopus subject areas
- Endocrinology
- Gastroenterology
Cite this
Gene expression analysis of pancreatic cell lines reveals genes overexpressed in pancreatic cancer. / Alldinger, Ingo; Dittert, Dag; Peiper, Matthias; Fusco, Alberto; Chiappetta, Gennaro; Staub, Eike; Löhr, Matthias; Jesnowski, Ralf; Baretton, Gustavo; Ockert, Detlef; Saeger, Hans Detlev; Grützmann, Robert; Pilarsky, Christian.
In: Pancreatology, Vol. 5, No. 4-5, 2005, p. 370-379.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Gene expression analysis of pancreatic cell lines reveals genes overexpressed in pancreatic cancer
AU - Alldinger, Ingo
AU - Dittert, Dag
AU - Peiper, Matthias
AU - Fusco, Alberto
AU - Chiappetta, Gennaro
AU - Staub, Eike
AU - Löhr, Matthias
AU - Jesnowski, Ralf
AU - Baretton, Gustavo
AU - Ockert, Detlef
AU - Saeger, Hans Detlev
AU - Grützmann, Robert
AU - Pilarsky, Christian
PY - 2005
Y1 - 2005
N2 - Background: Pancreatic cancer is one of the leading causes of cancer-related death. Using DNA gene expression analysis based on a custom made Affymetrix cancer array, we investigated the expression pattern of both primary and established pancreatic carcinoma cell lines. Methods: We analyzed the gene expression of 5 established pancreatic cancer cell lines (AsPC-1, BxPC-3, Capan-1, Capan-2 and HPAF II) and 5 primary isolates, 1 of them derived from benign pancreatic duct cells. Results: Out of 1,540 genes which were expressed in at least 3 experiments, we found 122 genes upregulated and 18 downregulated in tumor cell lines compared to benign cells with a fold change >3. Several of the upregulated genes (like Prefoldin 5, ADAM9 and E-cadherin) have been associated with pancreatic cancer before. The other differentially regulated genes, however, play a so far unknown role in the course of human pancreatic carcinoma. By means of immunohistochemistry we could show that thymosin β-10 (TMSB10), upregulated in tumor cell lines, is expressed in human pancreatic carcinoma, but not in non-neoplastic pancreatictissue, suggesting a role for TMSB10 in the carcinogenesis of pancreatic carcinoma. Conclusion: Using gene expression profiling of pancreatic cell lines we were able to identify genes differentially expressed in pancreatic adenocarcinoma, which might contribute to pancreatic cancer development.
AB - Background: Pancreatic cancer is one of the leading causes of cancer-related death. Using DNA gene expression analysis based on a custom made Affymetrix cancer array, we investigated the expression pattern of both primary and established pancreatic carcinoma cell lines. Methods: We analyzed the gene expression of 5 established pancreatic cancer cell lines (AsPC-1, BxPC-3, Capan-1, Capan-2 and HPAF II) and 5 primary isolates, 1 of them derived from benign pancreatic duct cells. Results: Out of 1,540 genes which were expressed in at least 3 experiments, we found 122 genes upregulated and 18 downregulated in tumor cell lines compared to benign cells with a fold change >3. Several of the upregulated genes (like Prefoldin 5, ADAM9 and E-cadherin) have been associated with pancreatic cancer before. The other differentially regulated genes, however, play a so far unknown role in the course of human pancreatic carcinoma. By means of immunohistochemistry we could show that thymosin β-10 (TMSB10), upregulated in tumor cell lines, is expressed in human pancreatic carcinoma, but not in non-neoplastic pancreatictissue, suggesting a role for TMSB10 in the carcinogenesis of pancreatic carcinoma. Conclusion: Using gene expression profiling of pancreatic cell lines we were able to identify genes differentially expressed in pancreatic adenocarcinoma, which might contribute to pancreatic cancer development.
KW - Cancer
KW - Cell line
KW - Immunohistochemistry
KW - Microarray
KW - Pancreas
KW - Primary isolates
KW - Thymosin
UR - http://www.scopus.com/inward/record.url?scp=23844519729&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=23844519729&partnerID=8YFLogxK
U2 - 10.1159/000086537
DO - 10.1159/000086537
M3 - Article
VL - 5
SP - 370
EP - 379
JO - Pancreatology
JF - Pancreatology
SN - 1424-3903
IS - 4-5
ER -