Gene expression analysis of pancreatic cell lines reveals genes overexpressed in pancreatic cancer

Ingo Alldinger, Dag Dittert, Matthias Peiper, Alberto Fusco, Gennaro Chiappetta, Eike Staub, Matthias Löhr, Ralf Jesnowski, Gustavo Baretton, Detlef Ockert, Hans Detlev Saeger, Robert Grützmann, Christian Pilarsky

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Background: Pancreatic cancer is one of the leading causes of cancer-related death. Using DNA gene expression analysis based on a custom made Affymetrix cancer array, we investigated the expression pattern of both primary and established pancreatic carcinoma cell lines. Methods: We analyzed the gene expression of 5 established pancreatic cancer cell lines (AsPC-1, BxPC-3, Capan-1, Capan-2 and HPAF II) and 5 primary isolates, 1 of them derived from benign pancreatic duct cells. Results: Out of 1,540 genes which were expressed in at least 3 experiments, we found 122 genes upregulated and 18 downregulated in tumor cell lines compared to benign cells with a fold change >3. Several of the upregulated genes (like Prefoldin 5, ADAM9 and E-cadherin) have been associated with pancreatic cancer before. The other differentially regulated genes, however, play a so far unknown role in the course of human pancreatic carcinoma. By means of immunohistochemistry we could show that thymosin β-10 (TMSB10), upregulated in tumor cell lines, is expressed in human pancreatic carcinoma, but not in non-neoplastic pancreatictissue, suggesting a role for TMSB10 in the carcinogenesis of pancreatic carcinoma. Conclusion: Using gene expression profiling of pancreatic cell lines we were able to identify genes differentially expressed in pancreatic adenocarcinoma, which might contribute to pancreatic cancer development.

Original languageEnglish
Pages (from-to)370-379
Number of pages10
JournalPancreatology
Volume5
Issue number4-5
DOIs
Publication statusPublished - 2005

Fingerprint

Pancreatic Neoplasms
Gene Expression
Cell Line
Genes
Tumor Cell Line
Thymosin
Pancreatic Ducts
Gene Expression Profiling
Cadherins
Neoplasms
Carcinogenesis
Adenocarcinoma
Down-Regulation
Immunohistochemistry
Pancreatic Carcinoma
DNA

Keywords

  • Cancer
  • Cell line
  • Immunohistochemistry
  • Microarray
  • Pancreas
  • Primary isolates
  • Thymosin

ASJC Scopus subject areas

  • Endocrinology
  • Gastroenterology

Cite this

Alldinger, I., Dittert, D., Peiper, M., Fusco, A., Chiappetta, G., Staub, E., ... Pilarsky, C. (2005). Gene expression analysis of pancreatic cell lines reveals genes overexpressed in pancreatic cancer. Pancreatology, 5(4-5), 370-379. https://doi.org/10.1159/000086537

Gene expression analysis of pancreatic cell lines reveals genes overexpressed in pancreatic cancer. / Alldinger, Ingo; Dittert, Dag; Peiper, Matthias; Fusco, Alberto; Chiappetta, Gennaro; Staub, Eike; Löhr, Matthias; Jesnowski, Ralf; Baretton, Gustavo; Ockert, Detlef; Saeger, Hans Detlev; Grützmann, Robert; Pilarsky, Christian.

In: Pancreatology, Vol. 5, No. 4-5, 2005, p. 370-379.

Research output: Contribution to journalArticle

Alldinger, I, Dittert, D, Peiper, M, Fusco, A, Chiappetta, G, Staub, E, Löhr, M, Jesnowski, R, Baretton, G, Ockert, D, Saeger, HD, Grützmann, R & Pilarsky, C 2005, 'Gene expression analysis of pancreatic cell lines reveals genes overexpressed in pancreatic cancer', Pancreatology, vol. 5, no. 4-5, pp. 370-379. https://doi.org/10.1159/000086537
Alldinger I, Dittert D, Peiper M, Fusco A, Chiappetta G, Staub E et al. Gene expression analysis of pancreatic cell lines reveals genes overexpressed in pancreatic cancer. Pancreatology. 2005;5(4-5):370-379. https://doi.org/10.1159/000086537
Alldinger, Ingo ; Dittert, Dag ; Peiper, Matthias ; Fusco, Alberto ; Chiappetta, Gennaro ; Staub, Eike ; Löhr, Matthias ; Jesnowski, Ralf ; Baretton, Gustavo ; Ockert, Detlef ; Saeger, Hans Detlev ; Grützmann, Robert ; Pilarsky, Christian. / Gene expression analysis of pancreatic cell lines reveals genes overexpressed in pancreatic cancer. In: Pancreatology. 2005 ; Vol. 5, No. 4-5. pp. 370-379.
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abstract = "Background: Pancreatic cancer is one of the leading causes of cancer-related death. Using DNA gene expression analysis based on a custom made Affymetrix cancer array, we investigated the expression pattern of both primary and established pancreatic carcinoma cell lines. Methods: We analyzed the gene expression of 5 established pancreatic cancer cell lines (AsPC-1, BxPC-3, Capan-1, Capan-2 and HPAF II) and 5 primary isolates, 1 of them derived from benign pancreatic duct cells. Results: Out of 1,540 genes which were expressed in at least 3 experiments, we found 122 genes upregulated and 18 downregulated in tumor cell lines compared to benign cells with a fold change >3. Several of the upregulated genes (like Prefoldin 5, ADAM9 and E-cadherin) have been associated with pancreatic cancer before. The other differentially regulated genes, however, play a so far unknown role in the course of human pancreatic carcinoma. By means of immunohistochemistry we could show that thymosin β-10 (TMSB10), upregulated in tumor cell lines, is expressed in human pancreatic carcinoma, but not in non-neoplastic pancreatictissue, suggesting a role for TMSB10 in the carcinogenesis of pancreatic carcinoma. Conclusion: Using gene expression profiling of pancreatic cell lines we were able to identify genes differentially expressed in pancreatic adenocarcinoma, which might contribute to pancreatic cancer development.",
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