OBJECTIVES: Tumour necrosis factor (TNF) receptor-associated periodic syndrome (TRAPS) is a multisystemic autoinflammatory condition associated with heterozygous TNFRSF1A mutations, presenting with a variety of clinical symptoms, many of which yet unexplained. In this work, we aimed at deepening into TRAPS pathogenic mechanisms sustained by monocytes.
METHODS: Microarray experiments were conducted to identify genes whose expression results altered in patients compared to healthy individuals, both under basal condition and following LPS stimulation.
RESULTS: An inflammatory state baseline, characterised by constitutive overexpression of IL1β and IL1R1 receptor, has been shown in TRAPS patients compared to controls, including in non-active disease phases. Following LPS stimulation, IL1RN up-regulation is stronger in controls than in patients and inflammatory pathways and microRNAs undergo differential regulation. Genes involved in post-translational modifications, protein folding and ubiquitination result constitutively up-regulated in TRAPS, while response to interferon types I and II is defective, failing to be up-regulated by LPS. TGFβ pathway is down-regulated in untreated TRAPS monocytes, while genes involved in redox regulation result constitutively over-expressed. Finally, additional molecular alterations seem to reflect organ failures sometime complicating the disease.
CONCLUSIONS: Gene expression profile in resting TRAPS monocytes has confirmed the patients' chronic inflammatory condition. In addition, pathways not yet associated with the disease have been disclosed, such as interferon types I and II response to LPS stimulation and a downregulation of the TGFβ pathway in basal condition. The role of miRNA, suggested by our results, deserves in-depth analyses in light of the possible development of targeted therapies.
|Journal||Clinical and Experimental Rheumatology|
|Issue number||6 Suppl 102|
|Publication status||Published - Oct 30 2016|
- Case-Control Studies
- Child, Preschool
- Gene Expression Profiling
- Gene Expression Regulation
- Genetic Association Studies
- Genetic Markers
- Genetic Predisposition to Disease
- Hereditary Autoinflammatory Diseases
- Inflammation Mediators
- Interleukin 1 Receptor Antagonist Protein
- Oligonucleotide Array Sequence Analysis
- Polymerase Chain Reaction
- Receptors, Tumor Necrosis Factor, Type I
- Reproducibility of Results
- Journal Article