Gene expression profile of peripheral blood mononuclear cells in response to HIV-VLPs stimulation

Luigi Buonaguro, Alessandro Monaco, Eleonora Aricò, Ena Wang, Maria Lina Lina, George K. Lewis, Franco M. Marincola, Franco M. Buonaguro

Research output: Contribution to journalArticle

Abstract

Background: Baculovirus-expressed HIV-1 Pr55gag Virus-Like Particles (HIV-VLPs) induce maturation and activation of monocyte-derived dendritic cells (MDDCs) with a production of Th1- and Th2-specific cytokines. Results: The analysis of genomic transcriptional profile of MDDCs, obtained from normal healthy donors and activated by HIV-VLPs, show the modulation of genes involved in the morphological and functional changes characterizing the MDDCs activation and maturation. Similar data are obtained using peripheral blood mononuclear cells (PBMCs), without further selection, showing the feasibility of a direct and "simplified" experimental procedure. Conclusions: The resultshere described show that the maturation pattern induced by HIV-VLPs in ex vivo generated MDDCs, can be observed also in CD14-expressing freshly derived PBMCs, with the possible identification of genetic predictors of individual response to immunogens.

Original languageEnglish
Article numberS5
JournalBMC Bioinformatics
Volume9
Issue numberSUPPL. 2
DOIs
Publication statusPublished - Mar 26 2008

ASJC Scopus subject areas

  • Medicine(all)
  • Structural Biology
  • Applied Mathematics

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