Gene expression profile of rat left ventricles reveals persisting changes following chronic mild exercise protocol: Implications for cardioprotection

Betti Giusti, Marina Marini, Luciana Rossi, Ilaria Lapini, Alberto Magi, Andrea Capalbo, Rosa Lapalombella, Simona di Tullio, Michele Samaja, Fabio Esposito, Vittoria Margonato, Maria Boddi, Rosanna Abbate, Arsenio Veicsteinas

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Abstract

Background: Epidemiological studies showed that physical exercise, specifically moderate lifelong training, is protective against cardiovascular morbidity and mortality. Most experimental work has focused into the effects and molecular mechanisms underlying intense, rather than mild exercise, by exploring the acute effect of training. Our study aims at investigating the cardioprotective effect of mild chronic exercise training and the gene expression profile changes at 48 hrs after the exercise cessation. Rats were trained at mild intensity on a treadmill: 25 m/min, 10%incline, 1 h/day, 3 days/week, 10 weeks; about 60% of the maximum aerobic power. By Affymetrix technology, we investigated the gene expression profile induced by exercise training in the left ventricle (LV) of trained (n = 10) and control (n = 10) rats. Cardioprotection was investigated by ischemia/reperfusion experiments (n = 10 trained vs. n = 10 control rats). Results: Mild exercise did not induce cardiac hypertrophy and was cardioprotective as demonstrated by the decreased infarct size (p = 0.02) after ischemia/reperfusion experiments in trained with respect to control rats. Ten genes and 2 gene sets (two pathways) resulted altered in LV of exercised animals with respect to controls. We validated by real-time PCR the increased expression of four genes: similar to C11orf17 protein (RGD1306959), caveolin 3, enolase 3, and hypoxia inducible factor 1 alpha. Moreover, caveolin 3 protein levels were higher in exercised than control rats by immunohistochemistry and Western Blot analysis. Interestingly, the predicted gene similar to C11orf17 protein (RGD1306959) was significantly increased by exercise. This gene has a high homology with the human C11orf17 (alias: protein kinase-A interacting protein 1 or breast cancer associated gene 3). This is the first evidence that this gene is involved in the response to the exercise training. Conclusion: Our data indicated that few, but significant, genes characterize the gene expression profile of the rat LV, when examined 48 hrs since the last training section and that mild exercise training determines cardioprotection without the induction of hypertrophy.

Original languageEnglish
Article number342
JournalBMC Genomics
Volume10
DOIs
Publication statusPublished - Jul 30 2009

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Transcriptome
Heart Ventricles
Exercise
Caveolin 3
Genes
Reperfusion
Ischemia
Caveolins
Hypoxia-Inducible Factor 1
Proteins
Phosphopyruvate Hydratase
Neoplasm Genes
Cardiomegaly
Cyclic AMP-Dependent Protein Kinases
Hypertrophy
Real-Time Polymerase Chain Reaction
Epidemiologic Studies
Breast
Western Blotting
Immunohistochemistry

ASJC Scopus subject areas

  • Biotechnology
  • Genetics

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Gene expression profile of rat left ventricles reveals persisting changes following chronic mild exercise protocol : Implications for cardioprotection. / Giusti, Betti; Marini, Marina; Rossi, Luciana; Lapini, Ilaria; Magi, Alberto; Capalbo, Andrea; Lapalombella, Rosa; di Tullio, Simona; Samaja, Michele; Esposito, Fabio; Margonato, Vittoria; Boddi, Maria; Abbate, Rosanna; Veicsteinas, Arsenio.

In: BMC Genomics, Vol. 10, 342, 30.07.2009.

Research output: Contribution to journalArticle

Giusti, B, Marini, M, Rossi, L, Lapini, I, Magi, A, Capalbo, A, Lapalombella, R, di Tullio, S, Samaja, M, Esposito, F, Margonato, V, Boddi, M, Abbate, R & Veicsteinas, A 2009, 'Gene expression profile of rat left ventricles reveals persisting changes following chronic mild exercise protocol: Implications for cardioprotection', BMC Genomics, vol. 10, 342. https://doi.org/10.1186/1471-2164-10-342
Giusti, Betti ; Marini, Marina ; Rossi, Luciana ; Lapini, Ilaria ; Magi, Alberto ; Capalbo, Andrea ; Lapalombella, Rosa ; di Tullio, Simona ; Samaja, Michele ; Esposito, Fabio ; Margonato, Vittoria ; Boddi, Maria ; Abbate, Rosanna ; Veicsteinas, Arsenio. / Gene expression profile of rat left ventricles reveals persisting changes following chronic mild exercise protocol : Implications for cardioprotection. In: BMC Genomics. 2009 ; Vol. 10.
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AU - Lapini, Ilaria

AU - Magi, Alberto

AU - Capalbo, Andrea

AU - Lapalombella, Rosa

AU - di Tullio, Simona

AU - Samaja, Michele

AU - Esposito, Fabio

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