Gene expression profiles in the cellular response to a multinuclear platinum complex

L. Gatti, G. L. Beretta, N. Carenini, E. Corna, F. Zunino, P. Perego

Research output: Contribution to journalArticlepeer-review

Abstract

We used cDNA arrays to monitor modulation of mRNA expression after exposure to a multinuclear platinum complex (BBR3464) in a human cervix squamous cell carcinoma cell line (A431) and in a cisplatin-resistant subline (A431/Pt) exhibiting collateral sensitivity to BBR3464. In parental A431 cells, the drug induced at least twofold up-regulation of 15 genes including cell cycle and growth regulators, tumor suppressors and signal transduction genes. In cisplatin-resistant A431/Pt cells, BBR3464 increased the expression of 15 genes such as apoptosis regulators and genes involved in the DNA damage response. Interestingly, BBR3464 induced up-regulation of anti-metastatic factors together with down-regulation of several pro-metastatic factors. Cell cycle analysis indicated a marked G2 arrest in treated A431 cells, whereas an apoptotic response was documented in A431/Pt cells. These differential patterns of transcriptional profile in sensitive and resistant cells are consistent with a role for cell cycle regulation in the response to BBR3464.

Original languageEnglish
Pages (from-to)973-981
Number of pages9
JournalCellular and Molecular Life Sciences
Volume61
Issue number7-8
DOIs
Publication statusPublished - Apr 2004

Keywords

  • cDNA array
  • Gene expression
  • Platinum compound
  • Resistance
  • Sensitivity

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

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