Gene expression profiles of epithelial cells microscopically isolated from a breast-invasive ductal carcinoma and a nodal metastasis

I. Zucchi, E. Mento, V. A. Kuznetsov, M. Scotti, V. Valsecchi, B. Simionati, E. Vicinanza, G. Valle, S. Pilotti, R. Reinbold, P. Vezzoni, A. Albertini, R. Dulbecco

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Expression profiles of breast carcinomas are difficult to interpret when they are obtained from tissue in toto, which may contain a large proportion of non-cancer cells. To avoid this problem, we microscopically isolated cells from a primary invasive ductal carcinoma of the breast and from an axillary node harboring a metastatic breast carcinoma, to obtain pure populations of carcinoma cells (≈500) and used them for serial analysis of gene expression. The expression profiles generated from both populations of cells were compared with the profile of a disease-free mammary epithelium. We showed that the expression profiles obtained are exclusive of carcinoma cells with no contribution of non-epithelial cells. From a total of 16,939 unique tags analyzed, we detected 559 statistically significant changes in gene expression; some of these genes have not been previously associated with breast cancer. We observed that many of the down-regulated genes are the same in both cancers, whereas the up-regulated genes are completely different, suggesting that the down-regulation of a set of genes may be the basic mechanism of cancer formation, while the up-regulation may characterize and possibly control the state of evolution of individual cancers. The results obtained may help in characterizing the neoplastic process of breast cancer.

Original languageEnglish
Pages (from-to)18147-18152
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number52
DOIs
Publication statusPublished - Dec 28 2004

Fingerprint

Carcinoma, Ductal, Breast
Transcriptome
Epithelial Cells
Neoplasm Metastasis
Breast Neoplasms
Genes
Carcinoma
Neoplastic Processes
Gene Expression
Neoplasms
Population
Breast
Up-Regulation
Down-Regulation
Epithelium

Keywords

  • Breast cancer
  • Carcinoma
  • Cell microdissection
  • Serial analysis of gene expression

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Gene expression profiles of epithelial cells microscopically isolated from a breast-invasive ductal carcinoma and a nodal metastasis. / Zucchi, I.; Mento, E.; Kuznetsov, V. A.; Scotti, M.; Valsecchi, V.; Simionati, B.; Vicinanza, E.; Valle, G.; Pilotti, S.; Reinbold, R.; Vezzoni, P.; Albertini, A.; Dulbecco, R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 52, 28.12.2004, p. 18147-18152.

Research output: Contribution to journalArticle

Zucchi, I, Mento, E, Kuznetsov, VA, Scotti, M, Valsecchi, V, Simionati, B, Vicinanza, E, Valle, G, Pilotti, S, Reinbold, R, Vezzoni, P, Albertini, A & Dulbecco, R 2004, 'Gene expression profiles of epithelial cells microscopically isolated from a breast-invasive ductal carcinoma and a nodal metastasis', Proceedings of the National Academy of Sciences of the United States of America, vol. 101, no. 52, pp. 18147-18152. https://doi.org/10.1073/pnas.0408260101
Zucchi, I. ; Mento, E. ; Kuznetsov, V. A. ; Scotti, M. ; Valsecchi, V. ; Simionati, B. ; Vicinanza, E. ; Valle, G. ; Pilotti, S. ; Reinbold, R. ; Vezzoni, P. ; Albertini, A. ; Dulbecco, R. / Gene expression profiles of epithelial cells microscopically isolated from a breast-invasive ductal carcinoma and a nodal metastasis. In: Proceedings of the National Academy of Sciences of the United States of America. 2004 ; Vol. 101, No. 52. pp. 18147-18152.
@article{9d90bb319d1c45069ee34ed2e6802f69,
title = "Gene expression profiles of epithelial cells microscopically isolated from a breast-invasive ductal carcinoma and a nodal metastasis",
abstract = "Expression profiles of breast carcinomas are difficult to interpret when they are obtained from tissue in toto, which may contain a large proportion of non-cancer cells. To avoid this problem, we microscopically isolated cells from a primary invasive ductal carcinoma of the breast and from an axillary node harboring a metastatic breast carcinoma, to obtain pure populations of carcinoma cells (≈500) and used them for serial analysis of gene expression. The expression profiles generated from both populations of cells were compared with the profile of a disease-free mammary epithelium. We showed that the expression profiles obtained are exclusive of carcinoma cells with no contribution of non-epithelial cells. From a total of 16,939 unique tags analyzed, we detected 559 statistically significant changes in gene expression; some of these genes have not been previously associated with breast cancer. We observed that many of the down-regulated genes are the same in both cancers, whereas the up-regulated genes are completely different, suggesting that the down-regulation of a set of genes may be the basic mechanism of cancer formation, while the up-regulation may characterize and possibly control the state of evolution of individual cancers. The results obtained may help in characterizing the neoplastic process of breast cancer.",
keywords = "Breast cancer, Carcinoma, Cell microdissection, Serial analysis of gene expression",
author = "I. Zucchi and E. Mento and Kuznetsov, {V. A.} and M. Scotti and V. Valsecchi and B. Simionati and E. Vicinanza and G. Valle and S. Pilotti and R. Reinbold and P. Vezzoni and A. Albertini and R. Dulbecco",
year = "2004",
month = "12",
day = "28",
doi = "10.1073/pnas.0408260101",
language = "English",
volume = "101",
pages = "18147--18152",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "52",

}

TY - JOUR

T1 - Gene expression profiles of epithelial cells microscopically isolated from a breast-invasive ductal carcinoma and a nodal metastasis

AU - Zucchi, I.

AU - Mento, E.

AU - Kuznetsov, V. A.

AU - Scotti, M.

AU - Valsecchi, V.

AU - Simionati, B.

AU - Vicinanza, E.

AU - Valle, G.

AU - Pilotti, S.

AU - Reinbold, R.

AU - Vezzoni, P.

AU - Albertini, A.

AU - Dulbecco, R.

PY - 2004/12/28

Y1 - 2004/12/28

N2 - Expression profiles of breast carcinomas are difficult to interpret when they are obtained from tissue in toto, which may contain a large proportion of non-cancer cells. To avoid this problem, we microscopically isolated cells from a primary invasive ductal carcinoma of the breast and from an axillary node harboring a metastatic breast carcinoma, to obtain pure populations of carcinoma cells (≈500) and used them for serial analysis of gene expression. The expression profiles generated from both populations of cells were compared with the profile of a disease-free mammary epithelium. We showed that the expression profiles obtained are exclusive of carcinoma cells with no contribution of non-epithelial cells. From a total of 16,939 unique tags analyzed, we detected 559 statistically significant changes in gene expression; some of these genes have not been previously associated with breast cancer. We observed that many of the down-regulated genes are the same in both cancers, whereas the up-regulated genes are completely different, suggesting that the down-regulation of a set of genes may be the basic mechanism of cancer formation, while the up-regulation may characterize and possibly control the state of evolution of individual cancers. The results obtained may help in characterizing the neoplastic process of breast cancer.

AB - Expression profiles of breast carcinomas are difficult to interpret when they are obtained from tissue in toto, which may contain a large proportion of non-cancer cells. To avoid this problem, we microscopically isolated cells from a primary invasive ductal carcinoma of the breast and from an axillary node harboring a metastatic breast carcinoma, to obtain pure populations of carcinoma cells (≈500) and used them for serial analysis of gene expression. The expression profiles generated from both populations of cells were compared with the profile of a disease-free mammary epithelium. We showed that the expression profiles obtained are exclusive of carcinoma cells with no contribution of non-epithelial cells. From a total of 16,939 unique tags analyzed, we detected 559 statistically significant changes in gene expression; some of these genes have not been previously associated with breast cancer. We observed that many of the down-regulated genes are the same in both cancers, whereas the up-regulated genes are completely different, suggesting that the down-regulation of a set of genes may be the basic mechanism of cancer formation, while the up-regulation may characterize and possibly control the state of evolution of individual cancers. The results obtained may help in characterizing the neoplastic process of breast cancer.

KW - Breast cancer

KW - Carcinoma

KW - Cell microdissection

KW - Serial analysis of gene expression

UR - http://www.scopus.com/inward/record.url?scp=19944427504&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=19944427504&partnerID=8YFLogxK

U2 - 10.1073/pnas.0408260101

DO - 10.1073/pnas.0408260101

M3 - Article

C2 - 15608061

AN - SCOPUS:19944427504

VL - 101

SP - 18147

EP - 18152

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 52

ER -