Gene expression profiling in skeletal muscle using a dedicated microarray

S. Campanaro, C. Romualdi, C. De Pittà, M. Fanin, B. Celegato, B. Pacchioni, S. Trevisan, P. Laveder, S. Toppo, S. Cagnin, G. Valle, C. Angelini, G. Lanfranchi

Research output: Contribution to journalArticle

Abstract

Background. Microarrays can measure simultaneously the expression of thousands of genes and identify subtle changes in expression between different biological states. In our lab a novel two-step subtractive strategy was settled in order to enrich for genes expressed at low levels in skeletal muscle. Methods. Using this cDNA collection we have developed a dedicated muscle-specific microarray composed of 2,700 sequences corresponding to the 400-500 most 3′-terminal region of muscle transcripts. Results. The expression profile of limb-girdle dystrophy type 2B was analysed with our array. Conclusions. We found approximately 3% differentially expressed genes, mostly involved in muscle contraction and immune response.

Original languageEnglish
Pages (from-to)273-279
Number of pages7
JournalMinerva Biotecnologica
Volume14
Issue number3-4
Publication statusPublished - Dec 2002

Keywords

  • DNA microarray
  • Gene expression
  • Muscular dystrophies
  • Skeletal muscle
  • Subtraction library

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

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  • Cite this

    Campanaro, S., Romualdi, C., De Pittà, C., Fanin, M., Celegato, B., Pacchioni, B., Trevisan, S., Laveder, P., Toppo, S., Cagnin, S., Valle, G., Angelini, C., & Lanfranchi, G. (2002). Gene expression profiling in skeletal muscle using a dedicated microarray. Minerva Biotecnologica, 14(3-4), 273-279.