Abstract
The relationship between inborn maximal oxygen uptake (VO2max) and skeletal muscle gene expression is unknown. Since low VO2max is a strong predictor of cardiovascular mortality, genes related to low VO 2max might also be involved in cardiovascular disease. To establish the relationship between inborn VO2max and gene expression, we performed microarray analysis of the soleus muscle of rats artificially selected for high- and low running capacity (HCR and LCR, respectively). In LCR, a low VO2max was accompanied by aggregation of cardiovascular risk factors similar to the metabolic syndrome. Although sedentary HCR were able to maintain a 120% higher running speed at VO2max than sedentary LCR, only three transcripts were differentially expressed (FDR ≤ 0.05) between the groups. Sedentary LCR expressed high levels of a transcript with strong homology to human leucyl-transfer RNA synthetase, of whose overexpression has been associated with a mutation linked to mitochondrial dysfunction. Moreover, we studied exercise-induced alterations in soleus gene expression, since accumulating evidence indicates that long-term endurance training has beneficial effects on the metabolic syndrome. In terms of gene expression, the response to exercise training was more pronounced in HCR than LCR. HCR upregulated several genes associated with lipid metabolism and fatty acid elongation, whereas LCR upregulated only one transcript after exercise training. The results indicate only minor differences in soleus muscle gene expression between sedentary HCR and LCR. However, the inborn level of fitness seems to influence the transcriptional adaption to exercise, as more genes were upregulated after exercise training in HCR than LCR.
| Original language | English |
|---|---|
| Pages (from-to) | 213-221 |
| Number of pages | 9 |
| Journal | Physiol Genomics |
| Volume | 35 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Nov 2008 |
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Keywords
- Gene ontology
- MELAS
- Metabolic syndrome
- Microarray analysis
- Soleus muscle
ASJC Scopus subject areas
- Physiology
- Genetics
Cite this
Gene expression profiling of skeletal muscle in exercise-trained and sedentary rats with inborn high and low VO2max. / Bye, Anja; Høydal, Morten A.; Catalucci, Daniele; Langaas, Mette; Kemi, Ole Johan; Beisvag, Vidar; Koch, Lauren G.; Britton, Steven L.; Ellingsen, Øyvind; Wisløff, Ulrik.
In: Physiol Genomics, Vol. 35, No. 3, 11.2008, p. 213-221.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Gene expression profiling of skeletal muscle in exercise-trained and sedentary rats with inborn high and low VO2max
AU - Bye, Anja
AU - Høydal, Morten A.
AU - Catalucci, Daniele
AU - Langaas, Mette
AU - Kemi, Ole Johan
AU - Beisvag, Vidar
AU - Koch, Lauren G.
AU - Britton, Steven L.
AU - Ellingsen, Øyvind
AU - Wisløff, Ulrik
PY - 2008/11
Y1 - 2008/11
N2 - The relationship between inborn maximal oxygen uptake (VO2max) and skeletal muscle gene expression is unknown. Since low VO2max is a strong predictor of cardiovascular mortality, genes related to low VO 2max might also be involved in cardiovascular disease. To establish the relationship between inborn VO2max and gene expression, we performed microarray analysis of the soleus muscle of rats artificially selected for high- and low running capacity (HCR and LCR, respectively). In LCR, a low VO2max was accompanied by aggregation of cardiovascular risk factors similar to the metabolic syndrome. Although sedentary HCR were able to maintain a 120% higher running speed at VO2max than sedentary LCR, only three transcripts were differentially expressed (FDR ≤ 0.05) between the groups. Sedentary LCR expressed high levels of a transcript with strong homology to human leucyl-transfer RNA synthetase, of whose overexpression has been associated with a mutation linked to mitochondrial dysfunction. Moreover, we studied exercise-induced alterations in soleus gene expression, since accumulating evidence indicates that long-term endurance training has beneficial effects on the metabolic syndrome. In terms of gene expression, the response to exercise training was more pronounced in HCR than LCR. HCR upregulated several genes associated with lipid metabolism and fatty acid elongation, whereas LCR upregulated only one transcript after exercise training. The results indicate only minor differences in soleus muscle gene expression between sedentary HCR and LCR. However, the inborn level of fitness seems to influence the transcriptional adaption to exercise, as more genes were upregulated after exercise training in HCR than LCR.
AB - The relationship between inborn maximal oxygen uptake (VO2max) and skeletal muscle gene expression is unknown. Since low VO2max is a strong predictor of cardiovascular mortality, genes related to low VO 2max might also be involved in cardiovascular disease. To establish the relationship between inborn VO2max and gene expression, we performed microarray analysis of the soleus muscle of rats artificially selected for high- and low running capacity (HCR and LCR, respectively). In LCR, a low VO2max was accompanied by aggregation of cardiovascular risk factors similar to the metabolic syndrome. Although sedentary HCR were able to maintain a 120% higher running speed at VO2max than sedentary LCR, only three transcripts were differentially expressed (FDR ≤ 0.05) between the groups. Sedentary LCR expressed high levels of a transcript with strong homology to human leucyl-transfer RNA synthetase, of whose overexpression has been associated with a mutation linked to mitochondrial dysfunction. Moreover, we studied exercise-induced alterations in soleus gene expression, since accumulating evidence indicates that long-term endurance training has beneficial effects on the metabolic syndrome. In terms of gene expression, the response to exercise training was more pronounced in HCR than LCR. HCR upregulated several genes associated with lipid metabolism and fatty acid elongation, whereas LCR upregulated only one transcript after exercise training. The results indicate only minor differences in soleus muscle gene expression between sedentary HCR and LCR. However, the inborn level of fitness seems to influence the transcriptional adaption to exercise, as more genes were upregulated after exercise training in HCR than LCR.
KW - Gene ontology
KW - MELAS
KW - Metabolic syndrome
KW - Microarray analysis
KW - Soleus muscle
UR - http://www.scopus.com/inward/record.url?scp=57449091092&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=57449091092&partnerID=8YFLogxK
U2 - 10.1152/physiolgenomics.90282.2008
DO - 10.1152/physiolgenomics.90282.2008
M3 - Article
C2 - 18780757
AN - SCOPUS:57449091092
VL - 35
SP - 213
EP - 221
JO - Physiological Genomics
JF - Physiological Genomics
SN - 1094-8341
IS - 3
ER -