TY - JOUR
T1 - Gene product immunophenotyping of neuroendocrine lung tumors
T2 - No linking evidence between carcinoids and small-cell lung carcinomas suggested by multivariate statistical analysis
AU - Sampietro, Giuseppe
AU - Tomasic, Gorana
AU - Collini, Paola
AU - Biganzoli, Elia
AU - Boracchi, Patrizia
AU - Bidoli, Paolo
AU - Pilotti, Silvana
PY - 2000
Y1 - 2000
N2 - Fifty-three neuroendocrine lung tumors (24 carcinoids, one atypical carcinoid, five large-cell neuroendocrine carcinomas, and 23 small-cell lung carcinomas) were investigated for immunocytochemical expression of several gene products, i.e., p53, Rb, bcl-2, c-kit, mdm-2, cdk-4, p21 proteins, and proliferation index as assessed by MIB-1. The goal of the study was to explore the relationships between histotypes in light of their own gene product-based immunophenotypical profiles. To this aim we applied the multiple correspondence analysis, which is an exploratory statistical multivariate technique that converts a data matrix into a particular type of graphic display in which the rows and columns are depicted as points. Such statistical analysis displayed that some categories of the gene product-based immunophenotyping variables are grouped in the plot identifying three groups: the first group related to carcinoids, the second to small-cell carcinomas, and the third to large-cell neuroendocrine carcinomas. These data support the evidence that carcinoids and small-cell carcinomas are two distinct, apparently immunogenotypically unrelated entities among neuroendocrine lung tumors and that atypical carcinoids and large-cell neuroendocrine carcinomas seem not to represent intermediate steps between them.
AB - Fifty-three neuroendocrine lung tumors (24 carcinoids, one atypical carcinoid, five large-cell neuroendocrine carcinomas, and 23 small-cell lung carcinomas) were investigated for immunocytochemical expression of several gene products, i.e., p53, Rb, bcl-2, c-kit, mdm-2, cdk-4, p21 proteins, and proliferation index as assessed by MIB-1. The goal of the study was to explore the relationships between histotypes in light of their own gene product-based immunophenotypical profiles. To this aim we applied the multiple correspondence analysis, which is an exploratory statistical multivariate technique that converts a data matrix into a particular type of graphic display in which the rows and columns are depicted as points. Such statistical analysis displayed that some categories of the gene product-based immunophenotyping variables are grouped in the plot identifying three groups: the first group related to carcinoids, the second to small-cell carcinomas, and the third to large-cell neuroendocrine carcinomas. These data support the evidence that carcinoids and small-cell carcinomas are two distinct, apparently immunogenotypically unrelated entities among neuroendocrine lung tumors and that atypical carcinoids and large-cell neuroendocrine carcinomas seem not to represent intermediate steps between them.
KW - Gene products
KW - Immunocytochemistry
KW - Multiple correspondence analysis
KW - Neuroendocrine lung tumors
KW - Statistical analysis
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U2 - 10.1097/00022744-200003000-00008
DO - 10.1097/00022744-200003000-00008
M3 - Article
C2 - 10937049
AN - SCOPUS:0034098759
VL - 8
SP - 49
EP - 56
JO - Applied Immunohistochemistry
JF - Applied Immunohistochemistry
SN - 1541-2016
IS - 1
ER -