Gene profiling and bioinformatic analysis of Schwann cell embryonic development and myelination

Maurizio D'Antonio, David Michalovich, Morris Paterson, Anna Droggiti, Ashwin Woodhoo, Rhona Mirsky, Kristjan R. Jessen

Research output: Contribution to journalArticle

Abstract

To elucidate the molecular mechanisms involved in Schwann cell development, we profiled gene expression in the developing and injured rat sciatic nerve. The genes that showed significant changes in expression in developing and dedifferentiated nerve were validated with RT-PCR, in situ hybridisation, Western blot and immunofluorescence. A comprehensive approach to annotating micro-array probes and their associated transcripts was performed using Biopendium™, a database of sequence and structural annotation. This approach significantly increased the number of genes for which a functional insight could be found. The analysis implicates agrin and two members of the collapsin response-mediated protein (CRMP) family in the switch from precursors to Schwann cells, and synuclein-1 and αB-crystallin in peripheral nerve myelination. We also identified a group of genes typically related to chondrogenesis and cartilage/bone development, including type II collagen, that were expressed in a manner similar to that of myelin-associated genes. The comprehensive function annotation also identified, among the genes regulated during nerve development or after nerve injury, proteins belonging to high-interest families, such as cytokines and kinases, and should therefore provide a uniquely valuable resource for future research.

Original languageEnglish
Pages (from-to)501-515
Number of pages15
JournalGLIA
Volume53
Issue number5
DOIs
Publication statusPublished - Apr 1 2006

Keywords

  • Collagen type II
  • Microarray
  • Myelin
  • Synuclein

ASJC Scopus subject areas

  • Immunology

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    D'Antonio, M., Michalovich, D., Paterson, M., Droggiti, A., Woodhoo, A., Mirsky, R., & Jessen, K. R. (2006). Gene profiling and bioinformatic analysis of Schwann cell embryonic development and myelination. GLIA, 53(5), 501-515. https://doi.org/10.1002/glia.20309