Gene silencing by S-adenosylmethionine in muscle differentiation

Andrea Fuso, Rosaria A. Cavallaro, Luigi Orrù, Francesca R. Buttarelli, Sigfrido Scarpa

Research output: Contribution to journalArticlepeer-review

Abstract

A well-characterised experimental system, the myogenin gene in C2C12 muscle cell culture, was chosen to better understand the methylation mechanism underlying the regulation of gene expression. We already demonstrated that demethylation dynamics of a specific CpG site in the 5′-flanking region of myogenin well correlates with gene expression and terminal differentiation. Here we demonstrate that S-adenosylmethionine-sulphate-p-toluenesulphonate (SAM) inhibits myogenin expression and myoblast differentiation by delaying the demethylation of specific CpG in differentiating myoblasts. These results suggest new perspectives in methylation mechanisms and the use of SAM in the partial silencing of gene expression, as it could be required in disease treatment.

Original languageEnglish
Pages (from-to)337-340
Number of pages4
JournalFEBS Letters
Volume508
Issue number3
DOIs
Publication statusPublished - Nov 23 2001

Keywords

  • DNA methylation
  • Gene silencing
  • Muscle differentiation
  • S-Adenosylmethionine cycle
  • Transcription regulation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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