Gene transfection efficiency of tracheal epithelial cells by DC-Chol-DOPE/DNA complexes

Alessia Colosimo, Annalucia Serafino, Federica Sangiuolo, Sabrina Di Sario, Emanuela Bruscia, Paola Amicucci, Giuseppe Novelli, Bruno Dallapiccola, Giuseppe Mossa

Research output: Contribution to journalArticlepeer-review

Abstract

We evaluated the transfection efficiency of five different cationic liposome/plasmid DNA complexes, during the in vitro gene transfer into human epithelial tracheal cell lines. A dramatic correlation between the transfection efficiency and the charge ratio (positive charge of liposome to negative charge of DNA) has been found. DC-Chol-DOPE was found to be the most effective liposome formulation. Therefore, a morphological and structural analysis of DC-Chol-DOPE liposomes and DC-Chol-DOPE/DNA complexes, has been performed by transmission electron microscopy (TEM) and by confocal laser scanning microscopy (CLSM), respectively. The process of interaction between DC-Chol-DOPE/DNA complexes and human epithelial tracheal cells has been studied by CLSM. These results raise some issues for in vivo gene therapy. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)186-194
Number of pages9
JournalBBA - Biomembranes
Volume1419
Issue number2
DOIs
Publication statusPublished - Jul 15 1999

Keywords

  • Cationic liposome
  • Gene therapy
  • Tracheal epithelial cell

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biophysics

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