TY - JOUR
T1 - Gene transfection efficiency of tracheal epithelial cells by DC-Chol-DOPE/DNA complexes
AU - Colosimo, Alessia
AU - Serafino, Annalucia
AU - Sangiuolo, Federica
AU - Di Sario, Sabrina
AU - Bruscia, Emanuela
AU - Amicucci, Paola
AU - Novelli, Giuseppe
AU - Dallapiccola, Bruno
AU - Mossa, Giuseppe
PY - 1999/7/15
Y1 - 1999/7/15
N2 - We evaluated the transfection efficiency of five different cationic liposome/plasmid DNA complexes, during the in vitro gene transfer into human epithelial tracheal cell lines. A dramatic correlation between the transfection efficiency and the charge ratio (positive charge of liposome to negative charge of DNA) has been found. DC-Chol-DOPE was found to be the most effective liposome formulation. Therefore, a morphological and structural analysis of DC-Chol-DOPE liposomes and DC-Chol-DOPE/DNA complexes, has been performed by transmission electron microscopy (TEM) and by confocal laser scanning microscopy (CLSM), respectively. The process of interaction between DC-Chol-DOPE/DNA complexes and human epithelial tracheal cells has been studied by CLSM. These results raise some issues for in vivo gene therapy. Copyright (C) 1999 Elsevier Science B.V.
AB - We evaluated the transfection efficiency of five different cationic liposome/plasmid DNA complexes, during the in vitro gene transfer into human epithelial tracheal cell lines. A dramatic correlation between the transfection efficiency and the charge ratio (positive charge of liposome to negative charge of DNA) has been found. DC-Chol-DOPE was found to be the most effective liposome formulation. Therefore, a morphological and structural analysis of DC-Chol-DOPE liposomes and DC-Chol-DOPE/DNA complexes, has been performed by transmission electron microscopy (TEM) and by confocal laser scanning microscopy (CLSM), respectively. The process of interaction between DC-Chol-DOPE/DNA complexes and human epithelial tracheal cells has been studied by CLSM. These results raise some issues for in vivo gene therapy. Copyright (C) 1999 Elsevier Science B.V.
KW - Cationic liposome
KW - Gene therapy
KW - Tracheal epithelial cell
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U2 - 10.1016/S0005-2736(99)00067-X
DO - 10.1016/S0005-2736(99)00067-X
M3 - Article
C2 - 10407070
AN - SCOPUS:0033067270
VL - 1419
SP - 186
EP - 194
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
SN - 0005-2736
IS - 2
ER -