Gene transfer to lentil protoplasts by lipofection and electroporation

Mauro Maccarrone, Luisa Di Marzio, Antonello Rossi, Alessandro Finazzi Agrò

Research output: Contribution to journalArticle

Abstract

Cationic liposomes made of dipalmitoylphosphatidylcholine and stearylamine (9:1) were prepared by reverse-phase evaporation and were able to interact spontaneously with plasmid DNA. The loaded vesicles delivered a βglucuronidase (GUS)-carrying plasmid to lentil Lens culinaris) protoplasts, leading to transient expression of the GUS reporter gene. The transfection efficiency achieved by using stearylamine-containing liposomes (lipofection) was comparable to the one obtained by electroporating the protoplasts at 500 μF and different field strengths. Furthermore, the combination of electroporation and lipofection, though reducing cell survival, increased the activity of the reporter enzyme detected in the cell lysates, yielding transient expression levels higher than those recorded after lipofection or electroporation alone.

Original languageEnglish
Pages (from-to)707-716
Number of pages10
JournalJournal of Liposome Research
Volume3
Issue number3
DOIs
Publication statusPublished - 1993

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ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology

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