General transcription factors bind promoters repressed by Polycomb group proteins.

A. Breiling, B. M. Turner, M. E. Bianchi, V. Orlando

Research output: Contribution to journalArticle

195 Citations (Scopus)

Abstract

To maintain cell identity during development and differentiation, mechanisms of cellular memory have evolved that preserve transcription patterns in an epigenetic manner. The proteins of the Polycomb group (PcG) are part of such a mechanism, maintaining gene silencing. They act as repressive multiprotein complexes that may render target genes inaccessible to the transcriptional machinery, inhibit chromatin remodelling, influence chromosome domain topology and recruit histone deacetylases (HDACs). PcG proteins have also been found to bind to core promoter regions, but the mechanism by which they regulate transcription remains unknown. To address this, we used formaldehyde-crosslinked chromatin immunoprecipitation (X-ChIP) to map TATA-binding protein (TBP), transcription initiation factor IIB (TFIIB) and IIF (TFIIF), and dHDAC1 (RPD3) across several Drosophila promoter regions. Here we show that binding of PcG proteins to repressed promoters does not exclude general transcription factors (GTFs) and that depletion of PcG proteins by double-stranded RNA interference leads to de-repression of developmentally regulated genes. We further show that PcG proteins interact in vitro with GTFs. We suggest that PcG complexes maintain silencing by inhibiting GTF-mediated activation of transcription.

Original languageEnglish
Pages (from-to)651-655
Number of pages5
JournalNature
Volume412
Issue number6847
Publication statusPublished - Aug 9 2001

Fingerprint

Polycomb-Group Proteins
General Transcription Factors
Genetic Promoter Regions
Transcription Factor TFIIB
TATA-Box Binding Protein
Multiprotein Complexes
Peptide Initiation Factors
Histone Deacetylases
Chromatin Assembly and Disassembly
Double-Stranded RNA
Chromatin Immunoprecipitation
Gene Silencing
RNA Interference
Epigenomics
Formaldehyde
Transcriptional Activation
Genes
Drosophila
Chromosomes

ASJC Scopus subject areas

  • General

Cite this

Breiling, A., Turner, B. M., Bianchi, M. E., & Orlando, V. (2001). General transcription factors bind promoters repressed by Polycomb group proteins. Nature, 412(6847), 651-655.

General transcription factors bind promoters repressed by Polycomb group proteins. / Breiling, A.; Turner, B. M.; Bianchi, M. E.; Orlando, V.

In: Nature, Vol. 412, No. 6847, 09.08.2001, p. 651-655.

Research output: Contribution to journalArticle

Breiling, A, Turner, BM, Bianchi, ME & Orlando, V 2001, 'General transcription factors bind promoters repressed by Polycomb group proteins.', Nature, vol. 412, no. 6847, pp. 651-655.
Breiling, A. ; Turner, B. M. ; Bianchi, M. E. ; Orlando, V. / General transcription factors bind promoters repressed by Polycomb group proteins. In: Nature. 2001 ; Vol. 412, No. 6847. pp. 651-655.
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