Generating Multicompartmental 3D Biological Constructs Interfaced through Sequential Injections in Microfluidic Devices

Giovanni Stefano Ugolini, Roberta Visone, Alberto Redaelli, Matteo Moretti, Marco Rasponi

Research output: Contribution to journalArticle

Abstract

A novel technique is presented for molding and culturing composite 3D cellular constructs within microfluidic channels. The method is based on the use of removable molding polydimethylsiloxane (PDMS) inserts, which allow to selectively and incrementally generate composite 3D constructs featuring different cell types and/or biomaterials, with a high spatial control. The authors generate constructs made of either stacked hydrogels, with uniform horizontal interfaces, or flanked hydrogels with vertical interfaces. The authors also show how this technique can be employed to create custom-shaped endothelial barriers and monolayers directly interfaced with 3D cellular constructs. This method dramatically improves the significance of in vitro 3D biological models, enhancing mimicry and enabling for controlled studies of complex biological districts.

Original languageEnglish
Article number1601170
JournalAdvanced healthcare materials
Volume6
Issue number10
DOIs
Publication statusPublished - May 24 2017
Externally publishedYes

Keywords

  • biofabrication
  • composite 3D constructs
  • microfluidics
  • microtissues, organs-on-chip

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Pharmaceutical Science

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