Generation and characterization of a human chromosome 9 cosmid library

Sharon L. Graw, Alan J. Buckler, Deborah E. Britt, Cynthia L. Jackson, Domenica Taruscio, Antonio Baldini, David C. Ward, David E. Housman

Research output: Contribution to journalArticle

Abstract

A cosmid library has been constructed from the hamster-human hybrid cell line PK-87-9, which contains chromosome 9 as its sole known human component. Ten thousand colonies were produced, of which approximately 200, or 2%, contain human material. Fifty of these 200 were regionally mapped by an Alu-primed PCR product hybridization procedure. These cosmids were localized to all regions of chromosome 9, but were especially concentrated in the distal portion of 9q. The map location derived by the Alu-primed PCR product hybridization procedure was compared to the map location derived by fluorescent in situ hybridization. Assignment of chromosomal location by the two methods was correspondent in all but a few cases. The presumptive presence of HTF islands was investigated for 130 cosmids by digestion with the restriction enzyme NotI. Twenty percent of cosmids contained at least one NotI site. A number of simple sequence repeat polymorphisms identified from the cosmid set were characterized and will provide a link between the genetic and physical maps for this chromosome.

Original languageEnglish
Pages (from-to)269-284
Number of pages16
JournalSomatic Cell and Molecular Genetics
Volume18
Issue number3
DOIs
Publication statusPublished - May 1992

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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    Graw, S. L., Buckler, A. J., Britt, D. E., Jackson, C. L., Taruscio, D., Baldini, A., Ward, D. C., & Housman, D. E. (1992). Generation and characterization of a human chromosome 9 cosmid library. Somatic Cell and Molecular Genetics, 18(3), 269-284. https://doi.org/10.1007/BF01233863