Generation of cardiomyocytes from pluripotent stem cells

Hiroko Nakahama, Elisa Di Pasquale

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The advent of pluripotent stem cells (PSCs) enabled a multitude of studies for modeling the development of diseases and testing pharmaceutical therapeutic potential in vitro. These PSCs have been differentiated to multiple cell types to demonstrate its pluripotent potential, including cardiomyocytes (CMs). However, the efficiency and efficacy of differentiation vary greatly between different cell lines and methods. Here, we describe two different methods for acquiring CMs from human pluripotent lines. One method involves the generation of embryoid bodies, which emulates the natural developmental process, while the other method chemically activates the canonical Wnt signaling pathway to induce a monolayer of cardiac differentiation.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages181-190
Number of pages10
Volume1353
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1353
ISSN (Print)10643745

Keywords

  • Cardiac differentiation
  • EB method
  • Human cardiomyocytes
  • Monolayer method
  • Pluripotent stem cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Nakahama, H., & Di Pasquale, E. (2016). Generation of cardiomyocytes from pluripotent stem cells. In Methods in Molecular Biology (Vol. 1353, pp. 181-190). (Methods in Molecular Biology; Vol. 1353). Humana Press Inc.. https://doi.org/10.1007/7651_2014_173