Generation of CFU-C/suppressor T cells in vitro: An experimental model for immune-mediated marrow failure

A. Bacigalupo, M. Podesta, M. Mingari, L. Moretta, G. Piaggio, M. T. Van Lint, A. Durando, A. M. Marmont

Research output: Contribution to journalArticlepeer-review

Abstract

T cells were derived from the bone marrow of 8 healthy donors and fractionated, according to their receptors for the Fc fragment of IgG, into T(G)+ and T(G)- lymphocytes. These were then cocultured with autologous or allogeneic bone marrow cells in agar in the CFU-C assay. No significant suppression of colony formation could be detected. Total T, T(G)+, and T(G)- cells were then incubated for 18 hr with PWM, washed, and cocultured with bone marrow cells. PWM-treated T(G)- cells showed no significant CFU-C suppressor activity, whereas PWM-treated total T and T(G)+ cells inhibited colony formation of both autologous and allogeneic marrow cells. The supernatant of PWM-treated total T and T(G)+ cells also inhibited colony formation. PWM alone enhanced colony formation. The results of this study indicate that normal T cells can be activated in vitro to become CFU-C/suppressor cells after PWM stimulation, and that this effect is mediated by T cells with the Fc receptor for IgG.

Original languageEnglish
Pages (from-to)491-496
Number of pages6
JournalBlood
Volume57
Issue number3
Publication statusPublished - 1981

ASJC Scopus subject areas

  • Hematology

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