Generation of CFUc Suppressor T cells in vitro. II. Effect of PHA, PWM and Con-A on bone marrow and peripheral blood lymphocytes from healthy donors

M. Podesta, F. Frassoni, M. T. van Lint, G. Piaggio, A. Marmont, A. Bacigalupo

Research output: Contribution to journalArticlepeer-review

Abstract

T lymphocytes were derived by E-rosetting techniques from the peripheral blood and bone marrow of 12 healthy donors. Following incubation for 18 h with PWM, PHA, Con-A or culture medium (RPMI) alone, the lymphocytes were harvested by centrifugation and washed. Both lymphocytes and culture supernatants were tested for CFUc suppressor activity in semisolid bone marrow cultures. The results of this study indicate that T cells and supernatants from unstimulated cultures contained no CFUc suppressor activity, T cells and supernatants from cultures stimulated with PHA, PWM or Con-A significantly suppressed autologous and allogeneic bone marrow CFUc, there was no significant difference between the ability of bone marrow or peripheral blood T cells to inhibit CFUc, and mitogens alone had either no effect or a moderate enhancing activity on marrow CFUc. These findings suggest that generation of CFUc suppressor T cells occurs normally within a few hours of polyclonal T cell stimulation.

Original languageEnglish
Pages (from-to)256-262
Number of pages7
JournalExperimental Hematology
Volume10
Issue number3
Publication statusPublished - 1982

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

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