Generation of CFUc suppressor T cells in vitro. IV. Effect of time on the inhibitory activity of mitogen-primed normal T lymphocytes

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Abstract

Bone marrow and peripheral blood T cells were obtained from 15 normal individuals by E rosetting and cultured in round-bottomed microwells for 7 days in RPMI or in RPMI supplemented with mitogens (pokeweed mitogen, phytohemagglutinin or concanavalin A). Supernatants and cells were harvested on days 1,2,3,4 and 7 and co-cultured with normal marrow cells in semi-solid agar to test their CFU-c suppressor activity. The results of this study indicate that RPMI treated cells and their supernatants have no effect or an enhancing effect on CFU-c growth; all 3 mitogens generate CFU-c suppressor T cells on day 1 culture; the inhibitory activity is detectable until day 4 of culture, though overall reduced, and is completely lost on day 7; the trend for supernatants of mitogen-treated T cells is quite similar with a tendency to complete loss of the inhibitory effect on day 7. We interpret these data as indicating that T cells release a soluble inhibitor of CFU-c growth within a few hours from polyclonal activation, the production of which is either controlled or lost with time in culture.

Original languageEnglish
Pages (from-to)233-242
Number of pages10
JournalThymus
Volume4
Issue number4
Publication statusPublished - 1982

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Mitogens
T-Lymphocytes
Bone Marrow
Pokeweed Mitogens
Phytohemagglutinins
Growth
Concanavalin A
Agar
Blood Cells
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

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title = "Generation of CFUc suppressor T cells in vitro. IV. Effect of time on the inhibitory activity of mitogen-primed normal T lymphocytes",
abstract = "Bone marrow and peripheral blood T cells were obtained from 15 normal individuals by E rosetting and cultured in round-bottomed microwells for 7 days in RPMI or in RPMI supplemented with mitogens (pokeweed mitogen, phytohemagglutinin or concanavalin A). Supernatants and cells were harvested on days 1,2,3,4 and 7 and co-cultured with normal marrow cells in semi-solid agar to test their CFU-c suppressor activity. The results of this study indicate that RPMI treated cells and their supernatants have no effect or an enhancing effect on CFU-c growth; all 3 mitogens generate CFU-c suppressor T cells on day 1 culture; the inhibitory activity is detectable until day 4 of culture, though overall reduced, and is completely lost on day 7; the trend for supernatants of mitogen-treated T cells is quite similar with a tendency to complete loss of the inhibitory effect on day 7. We interpret these data as indicating that T cells release a soluble inhibitor of CFU-c growth within a few hours from polyclonal activation, the production of which is either controlled or lost with time in culture.",
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T1 - Generation of CFUc suppressor T cells in vitro. IV. Effect of time on the inhibitory activity of mitogen-primed normal T lymphocytes

AU - Podesta, M.

AU - Frassoni, F.

AU - Van Lint, M. T.

PY - 1982

Y1 - 1982

N2 - Bone marrow and peripheral blood T cells were obtained from 15 normal individuals by E rosetting and cultured in round-bottomed microwells for 7 days in RPMI or in RPMI supplemented with mitogens (pokeweed mitogen, phytohemagglutinin or concanavalin A). Supernatants and cells were harvested on days 1,2,3,4 and 7 and co-cultured with normal marrow cells in semi-solid agar to test their CFU-c suppressor activity. The results of this study indicate that RPMI treated cells and their supernatants have no effect or an enhancing effect on CFU-c growth; all 3 mitogens generate CFU-c suppressor T cells on day 1 culture; the inhibitory activity is detectable until day 4 of culture, though overall reduced, and is completely lost on day 7; the trend for supernatants of mitogen-treated T cells is quite similar with a tendency to complete loss of the inhibitory effect on day 7. We interpret these data as indicating that T cells release a soluble inhibitor of CFU-c growth within a few hours from polyclonal activation, the production of which is either controlled or lost with time in culture.

AB - Bone marrow and peripheral blood T cells were obtained from 15 normal individuals by E rosetting and cultured in round-bottomed microwells for 7 days in RPMI or in RPMI supplemented with mitogens (pokeweed mitogen, phytohemagglutinin or concanavalin A). Supernatants and cells were harvested on days 1,2,3,4 and 7 and co-cultured with normal marrow cells in semi-solid agar to test their CFU-c suppressor activity. The results of this study indicate that RPMI treated cells and their supernatants have no effect or an enhancing effect on CFU-c growth; all 3 mitogens generate CFU-c suppressor T cells on day 1 culture; the inhibitory activity is detectable until day 4 of culture, though overall reduced, and is completely lost on day 7; the trend for supernatants of mitogen-treated T cells is quite similar with a tendency to complete loss of the inhibitory effect on day 7. We interpret these data as indicating that T cells release a soluble inhibitor of CFU-c growth within a few hours from polyclonal activation, the production of which is either controlled or lost with time in culture.

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