Abstract
Bone marrow and peripheral blood T cells were obtained from 15 normal individuals by E rosetting and cultured in round-bottomed microwells for 7 days in RPMI or in RPMI supplemented with mitogens (pokeweed mitogen, phytohemagglutinin or concanavalin A). Supernatants and cells were harvested on days 1,2,3,4 and 7 and co-cultured with normal marrow cells in semi-solid agar to test their CFU-c suppressor activity. The results of this study indicate that RPMI treated cells and their supernatants have no effect or an enhancing effect on CFU-c growth; all 3 mitogens generate CFU-c suppressor T cells on day 1 culture; the inhibitory activity is detectable until day 4 of culture, though overall reduced, and is completely lost on day 7; the trend for supernatants of mitogen-treated T cells is quite similar with a tendency to complete loss of the inhibitory effect on day 7. We interpret these data as indicating that T cells release a soluble inhibitor of CFU-c growth within a few hours from polyclonal activation, the production of which is either controlled or lost with time in culture.
| Original language | English |
|---|---|
| Pages (from-to) | 233-242 |
| Number of pages | 10 |
| Journal | Thymus |
| Volume | 4 |
| Issue number | 4 |
| Publication status | Published - 1982 |
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ASJC Scopus subject areas
- Immunology
Cite this
Generation of CFUc suppressor T cells in vitro. IV. Effect of time on the inhibitory activity of mitogen-primed normal T lymphocytes. / Podesta, M.; Frassoni, F.; Van Lint, M. T.
In: Thymus, Vol. 4, No. 4, 1982, p. 233-242.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Generation of CFUc suppressor T cells in vitro. IV. Effect of time on the inhibitory activity of mitogen-primed normal T lymphocytes
AU - Podesta, M.
AU - Frassoni, F.
AU - Van Lint, M. T.
PY - 1982
Y1 - 1982
N2 - Bone marrow and peripheral blood T cells were obtained from 15 normal individuals by E rosetting and cultured in round-bottomed microwells for 7 days in RPMI or in RPMI supplemented with mitogens (pokeweed mitogen, phytohemagglutinin or concanavalin A). Supernatants and cells were harvested on days 1,2,3,4 and 7 and co-cultured with normal marrow cells in semi-solid agar to test their CFU-c suppressor activity. The results of this study indicate that RPMI treated cells and their supernatants have no effect or an enhancing effect on CFU-c growth; all 3 mitogens generate CFU-c suppressor T cells on day 1 culture; the inhibitory activity is detectable until day 4 of culture, though overall reduced, and is completely lost on day 7; the trend for supernatants of mitogen-treated T cells is quite similar with a tendency to complete loss of the inhibitory effect on day 7. We interpret these data as indicating that T cells release a soluble inhibitor of CFU-c growth within a few hours from polyclonal activation, the production of which is either controlled or lost with time in culture.
AB - Bone marrow and peripheral blood T cells were obtained from 15 normal individuals by E rosetting and cultured in round-bottomed microwells for 7 days in RPMI or in RPMI supplemented with mitogens (pokeweed mitogen, phytohemagglutinin or concanavalin A). Supernatants and cells were harvested on days 1,2,3,4 and 7 and co-cultured with normal marrow cells in semi-solid agar to test their CFU-c suppressor activity. The results of this study indicate that RPMI treated cells and their supernatants have no effect or an enhancing effect on CFU-c growth; all 3 mitogens generate CFU-c suppressor T cells on day 1 culture; the inhibitory activity is detectable until day 4 of culture, though overall reduced, and is completely lost on day 7; the trend for supernatants of mitogen-treated T cells is quite similar with a tendency to complete loss of the inhibitory effect on day 7. We interpret these data as indicating that T cells release a soluble inhibitor of CFU-c growth within a few hours from polyclonal activation, the production of which is either controlled or lost with time in culture.
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M3 - Article
VL - 4
SP - 233
EP - 242
JO - Thymus
JF - Thymus
SN - 0165-6090
IS - 4
ER -