Abstract
T derived colony inhibitory activity (Td/CIA) was obtained from unstimulated T cells from aplastic anemia patients (SAA), or from PWM primed normal T cells. Td/CIA suppressed CFU(C) growth of normal allogeneic marrow to less than 30% of expected growth. Td/CIA was then added to normal peripheral blood T and B cells, printed with PWM, to test whether it would interfere with in vitro immunoglobulin (Ig) production. When Td/CIA from normal T cells was added to cultures of T + B cells + PWM there was a 2-2.1-fold increase in Ig production. Similarly the addition of Td/CIA from SAA patients also resulted in a 1.4 up to 166-fold increase in Ig production. These results indicate that either (a) the targets for Td/CIA are expressed on hemopoietic but not on T and B cells, or (b) that Td/CIA inactivates an accessory cell which is essential for CFU(C) growth but not for the PWM driven in vitro B cell differentiation system.
| Original language | English |
|---|---|
| Pages (from-to) | 506-511 |
| Number of pages | 6 |
| Journal | Experimental Hematology |
| Volume | 11 |
| Issue number | 6 |
| Publication status | Published - 1983 |
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ASJC Scopus subject areas
- Cancer Research
- Cell Biology
- Genetics
- Hematology
- Oncology
- Transplantation
Cite this
Generation of CFU(C) suppressor T cells in vitro : VII. T derived colony inhibitory activity (Td/CIA) has no suppressor effect on in vitro immunoglobulin production. / Bacigalupo, A.; Mingari, M. C.; Melioli, G.
In: Experimental Hematology, Vol. 11, No. 6, 1983, p. 506-511.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Generation of CFU(C) suppressor T cells in vitro
T2 - VII. T derived colony inhibitory activity (Td/CIA) has no suppressor effect on in vitro immunoglobulin production
AU - Bacigalupo, A.
AU - Mingari, M. C.
AU - Melioli, G.
PY - 1983
Y1 - 1983
N2 - T derived colony inhibitory activity (Td/CIA) was obtained from unstimulated T cells from aplastic anemia patients (SAA), or from PWM primed normal T cells. Td/CIA suppressed CFU(C) growth of normal allogeneic marrow to less than 30% of expected growth. Td/CIA was then added to normal peripheral blood T and B cells, printed with PWM, to test whether it would interfere with in vitro immunoglobulin (Ig) production. When Td/CIA from normal T cells was added to cultures of T + B cells + PWM there was a 2-2.1-fold increase in Ig production. Similarly the addition of Td/CIA from SAA patients also resulted in a 1.4 up to 166-fold increase in Ig production. These results indicate that either (a) the targets for Td/CIA are expressed on hemopoietic but not on T and B cells, or (b) that Td/CIA inactivates an accessory cell which is essential for CFU(C) growth but not for the PWM driven in vitro B cell differentiation system.
AB - T derived colony inhibitory activity (Td/CIA) was obtained from unstimulated T cells from aplastic anemia patients (SAA), or from PWM primed normal T cells. Td/CIA suppressed CFU(C) growth of normal allogeneic marrow to less than 30% of expected growth. Td/CIA was then added to normal peripheral blood T and B cells, printed with PWM, to test whether it would interfere with in vitro immunoglobulin (Ig) production. When Td/CIA from normal T cells was added to cultures of T + B cells + PWM there was a 2-2.1-fold increase in Ig production. Similarly the addition of Td/CIA from SAA patients also resulted in a 1.4 up to 166-fold increase in Ig production. These results indicate that either (a) the targets for Td/CIA are expressed on hemopoietic but not on T and B cells, or (b) that Td/CIA inactivates an accessory cell which is essential for CFU(C) growth but not for the PWM driven in vitro B cell differentiation system.
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M3 - Article
VL - 11
SP - 506
EP - 511
JO - Experimental Hematology
JF - Experimental Hematology
SN - 0301-472X
IS - 6
ER -