Generation of CFU(C) suppressor T cells in vitro

VII. T derived colony inhibitory activity (Td/CIA) has no suppressor effect on in vitro immunoglobulin production

A. Bacigalupo, M. C. Mingari, G. Melioli

Research output: Contribution to journalArticle

Abstract

T derived colony inhibitory activity (Td/CIA) was obtained from unstimulated T cells from aplastic anemia patients (SAA), or from PWM primed normal T cells. Td/CIA suppressed CFU(C) growth of normal allogeneic marrow to less than 30% of expected growth. Td/CIA was then added to normal peripheral blood T and B cells, printed with PWM, to test whether it would interfere with in vitro immunoglobulin (Ig) production. When Td/CIA from normal T cells was added to cultures of T + B cells + PWM there was a 2-2.1-fold increase in Ig production. Similarly the addition of Td/CIA from SAA patients also resulted in a 1.4 up to 166-fold increase in Ig production. These results indicate that either (a) the targets for Td/CIA are expressed on hemopoietic but not on T and B cells, or (b) that Td/CIA inactivates an accessory cell which is essential for CFU(C) growth but not for the PWM driven in vitro B cell differentiation system.

Original languageEnglish
Pages (from-to)506-511
Number of pages6
JournalExperimental Hematology
Volume11
Issue number6
Publication statusPublished - 1983

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Immunoglobulins
T-Lymphocytes
B-Lymphocytes
Growth
Aplastic Anemia
Cell Differentiation
Blood Cells
Bone Marrow
In Vitro Techniques

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

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title = "Generation of CFU(C) suppressor T cells in vitro: VII. T derived colony inhibitory activity (Td/CIA) has no suppressor effect on in vitro immunoglobulin production",
abstract = "T derived colony inhibitory activity (Td/CIA) was obtained from unstimulated T cells from aplastic anemia patients (SAA), or from PWM primed normal T cells. Td/CIA suppressed CFU(C) growth of normal allogeneic marrow to less than 30{\%} of expected growth. Td/CIA was then added to normal peripheral blood T and B cells, printed with PWM, to test whether it would interfere with in vitro immunoglobulin (Ig) production. When Td/CIA from normal T cells was added to cultures of T + B cells + PWM there was a 2-2.1-fold increase in Ig production. Similarly the addition of Td/CIA from SAA patients also resulted in a 1.4 up to 166-fold increase in Ig production. These results indicate that either (a) the targets for Td/CIA are expressed on hemopoietic but not on T and B cells, or (b) that Td/CIA inactivates an accessory cell which is essential for CFU(C) growth but not for the PWM driven in vitro B cell differentiation system.",
author = "A. Bacigalupo and Mingari, {M. C.} and G. Melioli",
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T2 - VII. T derived colony inhibitory activity (Td/CIA) has no suppressor effect on in vitro immunoglobulin production

AU - Bacigalupo, A.

AU - Mingari, M. C.

AU - Melioli, G.

PY - 1983

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N2 - T derived colony inhibitory activity (Td/CIA) was obtained from unstimulated T cells from aplastic anemia patients (SAA), or from PWM primed normal T cells. Td/CIA suppressed CFU(C) growth of normal allogeneic marrow to less than 30% of expected growth. Td/CIA was then added to normal peripheral blood T and B cells, printed with PWM, to test whether it would interfere with in vitro immunoglobulin (Ig) production. When Td/CIA from normal T cells was added to cultures of T + B cells + PWM there was a 2-2.1-fold increase in Ig production. Similarly the addition of Td/CIA from SAA patients also resulted in a 1.4 up to 166-fold increase in Ig production. These results indicate that either (a) the targets for Td/CIA are expressed on hemopoietic but not on T and B cells, or (b) that Td/CIA inactivates an accessory cell which is essential for CFU(C) growth but not for the PWM driven in vitro B cell differentiation system.

AB - T derived colony inhibitory activity (Td/CIA) was obtained from unstimulated T cells from aplastic anemia patients (SAA), or from PWM primed normal T cells. Td/CIA suppressed CFU(C) growth of normal allogeneic marrow to less than 30% of expected growth. Td/CIA was then added to normal peripheral blood T and B cells, printed with PWM, to test whether it would interfere with in vitro immunoglobulin (Ig) production. When Td/CIA from normal T cells was added to cultures of T + B cells + PWM there was a 2-2.1-fold increase in Ig production. Similarly the addition of Td/CIA from SAA patients also resulted in a 1.4 up to 166-fold increase in Ig production. These results indicate that either (a) the targets for Td/CIA are expressed on hemopoietic but not on T and B cells, or (b) that Td/CIA inactivates an accessory cell which is essential for CFU(C) growth but not for the PWM driven in vitro B cell differentiation system.

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