TY - JOUR
T1 - Generation of cytotoxic cells in absence of blastogenesis by mouse leukemic cells in mixed cultures
AU - Collavo, D.
AU - Biasi, G.
AU - Colombatti, A.
AU - Chieco Bianchi, L.
PY - 1976
Y1 - 1976
N2 - Primary virus induced murine leukemias and transplantable leukemic cell lines, originally induced by virus, chemical or physical agents, failed to stimulate normal spleen cells differing at the H 2 complex or at Mls locus in the one way mixed leukocyte culture. Lack of stimulation seems at least partially due to a nonspecific inhibitory effect since in a 'three party' culture system the leukemic cells interfered with the mixed leukocyte reaction (MLR) of normal allogeneic cells. Characterization of the leukemic cell populations used, according to their T or B cell origin, disclosed that in most cases T cell markers were present. Also, no Ia antigens were detected on leukemic cells. Furthermore, using a 51Cr release assay, leukemic cells could sensitize allogeneic normal lymphocytes to kill specifically normal as well as leukemic targets, even when no MLR was detected in parallel cultures.
AB - Primary virus induced murine leukemias and transplantable leukemic cell lines, originally induced by virus, chemical or physical agents, failed to stimulate normal spleen cells differing at the H 2 complex or at Mls locus in the one way mixed leukocyte culture. Lack of stimulation seems at least partially due to a nonspecific inhibitory effect since in a 'three party' culture system the leukemic cells interfered with the mixed leukocyte reaction (MLR) of normal allogeneic cells. Characterization of the leukemic cell populations used, according to their T or B cell origin, disclosed that in most cases T cell markers were present. Also, no Ia antigens were detected on leukemic cells. Furthermore, using a 51Cr release assay, leukemic cells could sensitize allogeneic normal lymphocytes to kill specifically normal as well as leukemic targets, even when no MLR was detected in parallel cultures.
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M3 - Article
C2 - 137121
AN - SCOPUS:0017187035
VL - 6
SP - 612
EP - 618
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 9
ER -