Generation of functional podocytes from human induced pluripotent stem cells

Osele Ciampi, Roberto Iacone, Lorena Longaretti, Valentina Benedetti, Martin Graf, Maria Chiara Magnone, Christoph Patsch, Christodoulos Xinaris, Giuseppe Remuzzi, Ariela Benigni, Susanna Tomasoni

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Generating human podocytes in vitro could offer a unique opportunity to study human diseases. Here, we describe a simple and efficient protocol for obtaining functional podocytes in vitro from human induced pluripotent stem cells. Cells were exposed to a three-step protocol, which induced their differentiation into intermediate mesoderm, then into nephron progenitors and, finally, into mature podocytes. After differentiation, cells expressed the main podocyte markers, such as synaptopodin, WT1, α-Actinin-4, P-cadherin and nephrin at the protein and mRNA level, and showed the low proliferation rate typical of mature podocytes. Exposure to Angiotensin II significantly decreased the expression of podocyte genes and cells underwent cytoskeleton rearrangement. Cells were able to internalize albumin and self-assembled into chimeric 3D structures in combination with dissociated embryonic mouse kidney cells. Overall, these findings demonstrate the establishment of a robust protocol that, mimicking developmental stages, makes it possible to derive functional podocytes in vitro.

Original languageEnglish
Pages (from-to)130-139
Number of pages10
JournalStem Cell Research
Volume17
Issue number1
DOIs
Publication statusPublished - Jul 1 2016

Fingerprint

Induced Pluripotent Stem Cells
Podocytes
Actinin
Nephrons
Mesoderm
Cadherins
Cytoskeleton
Angiotensin II
Albumins
Kidney
Gene Expression
Messenger RNA

Keywords

  • Differentiation
  • Induced pluripotent stem cells
  • Nephron progenitors
  • Podocytes

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Medicine(all)

Cite this

Ciampi, O., Iacone, R., Longaretti, L., Benedetti, V., Graf, M., Magnone, M. C., ... Tomasoni, S. (2016). Generation of functional podocytes from human induced pluripotent stem cells. Stem Cell Research, 17(1), 130-139. https://doi.org/10.1016/j.scr.2016.06.001

Generation of functional podocytes from human induced pluripotent stem cells. / Ciampi, Osele; Iacone, Roberto; Longaretti, Lorena; Benedetti, Valentina; Graf, Martin; Magnone, Maria Chiara; Patsch, Christoph; Xinaris, Christodoulos; Remuzzi, Giuseppe; Benigni, Ariela; Tomasoni, Susanna.

In: Stem Cell Research, Vol. 17, No. 1, 01.07.2016, p. 130-139.

Research output: Contribution to journalArticle

Ciampi, O, Iacone, R, Longaretti, L, Benedetti, V, Graf, M, Magnone, MC, Patsch, C, Xinaris, C, Remuzzi, G, Benigni, A & Tomasoni, S 2016, 'Generation of functional podocytes from human induced pluripotent stem cells', Stem Cell Research, vol. 17, no. 1, pp. 130-139. https://doi.org/10.1016/j.scr.2016.06.001
Ciampi O, Iacone R, Longaretti L, Benedetti V, Graf M, Magnone MC et al. Generation of functional podocytes from human induced pluripotent stem cells. Stem Cell Research. 2016 Jul 1;17(1):130-139. https://doi.org/10.1016/j.scr.2016.06.001
Ciampi, Osele ; Iacone, Roberto ; Longaretti, Lorena ; Benedetti, Valentina ; Graf, Martin ; Magnone, Maria Chiara ; Patsch, Christoph ; Xinaris, Christodoulos ; Remuzzi, Giuseppe ; Benigni, Ariela ; Tomasoni, Susanna. / Generation of functional podocytes from human induced pluripotent stem cells. In: Stem Cell Research. 2016 ; Vol. 17, No. 1. pp. 130-139.
@article{aea56c32133e4345bb55bec1b50d9ab8,
title = "Generation of functional podocytes from human induced pluripotent stem cells",
abstract = "Generating human podocytes in vitro could offer a unique opportunity to study human diseases. Here, we describe a simple and efficient protocol for obtaining functional podocytes in vitro from human induced pluripotent stem cells. Cells were exposed to a three-step protocol, which induced their differentiation into intermediate mesoderm, then into nephron progenitors and, finally, into mature podocytes. After differentiation, cells expressed the main podocyte markers, such as synaptopodin, WT1, α-Actinin-4, P-cadherin and nephrin at the protein and mRNA level, and showed the low proliferation rate typical of mature podocytes. Exposure to Angiotensin II significantly decreased the expression of podocyte genes and cells underwent cytoskeleton rearrangement. Cells were able to internalize albumin and self-assembled into chimeric 3D structures in combination with dissociated embryonic mouse kidney cells. Overall, these findings demonstrate the establishment of a robust protocol that, mimicking developmental stages, makes it possible to derive functional podocytes in vitro.",
keywords = "Differentiation, Induced pluripotent stem cells, Nephron progenitors, Podocytes",
author = "Osele Ciampi and Roberto Iacone and Lorena Longaretti and Valentina Benedetti and Martin Graf and Magnone, {Maria Chiara} and Christoph Patsch and Christodoulos Xinaris and Giuseppe Remuzzi and Ariela Benigni and Susanna Tomasoni",
year = "2016",
month = "7",
day = "1",
doi = "10.1016/j.scr.2016.06.001",
language = "English",
volume = "17",
pages = "130--139",
journal = "Stem Cell Research",
issn = "1873-5061",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Generation of functional podocytes from human induced pluripotent stem cells

AU - Ciampi, Osele

AU - Iacone, Roberto

AU - Longaretti, Lorena

AU - Benedetti, Valentina

AU - Graf, Martin

AU - Magnone, Maria Chiara

AU - Patsch, Christoph

AU - Xinaris, Christodoulos

AU - Remuzzi, Giuseppe

AU - Benigni, Ariela

AU - Tomasoni, Susanna

PY - 2016/7/1

Y1 - 2016/7/1

N2 - Generating human podocytes in vitro could offer a unique opportunity to study human diseases. Here, we describe a simple and efficient protocol for obtaining functional podocytes in vitro from human induced pluripotent stem cells. Cells were exposed to a three-step protocol, which induced their differentiation into intermediate mesoderm, then into nephron progenitors and, finally, into mature podocytes. After differentiation, cells expressed the main podocyte markers, such as synaptopodin, WT1, α-Actinin-4, P-cadherin and nephrin at the protein and mRNA level, and showed the low proliferation rate typical of mature podocytes. Exposure to Angiotensin II significantly decreased the expression of podocyte genes and cells underwent cytoskeleton rearrangement. Cells were able to internalize albumin and self-assembled into chimeric 3D structures in combination with dissociated embryonic mouse kidney cells. Overall, these findings demonstrate the establishment of a robust protocol that, mimicking developmental stages, makes it possible to derive functional podocytes in vitro.

AB - Generating human podocytes in vitro could offer a unique opportunity to study human diseases. Here, we describe a simple and efficient protocol for obtaining functional podocytes in vitro from human induced pluripotent stem cells. Cells were exposed to a three-step protocol, which induced their differentiation into intermediate mesoderm, then into nephron progenitors and, finally, into mature podocytes. After differentiation, cells expressed the main podocyte markers, such as synaptopodin, WT1, α-Actinin-4, P-cadherin and nephrin at the protein and mRNA level, and showed the low proliferation rate typical of mature podocytes. Exposure to Angiotensin II significantly decreased the expression of podocyte genes and cells underwent cytoskeleton rearrangement. Cells were able to internalize albumin and self-assembled into chimeric 3D structures in combination with dissociated embryonic mouse kidney cells. Overall, these findings demonstrate the establishment of a robust protocol that, mimicking developmental stages, makes it possible to derive functional podocytes in vitro.

KW - Differentiation

KW - Induced pluripotent stem cells

KW - Nephron progenitors

KW - Podocytes

UR - http://www.scopus.com/inward/record.url?scp=84973450025&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84973450025&partnerID=8YFLogxK

U2 - 10.1016/j.scr.2016.06.001

DO - 10.1016/j.scr.2016.06.001

M3 - Article

AN - SCOPUS:84973450025

VL - 17

SP - 130

EP - 139

JO - Stem Cell Research

JF - Stem Cell Research

SN - 1873-5061

IS - 1

ER -