Generation of murine growth factor-dependent long-term dendritic cell lines to investigate host-parasite interactions

Alessandra Mortellaro, Matteo Urbano, Stefania Citterio, Maria Foti, Francesca Granucci, Paola Ricciardi-Castagnoli

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Substantial progress has been made over the last several years in the development of protocols for the isolation of large numbers of dendritic cells (DCs) from different tissues and their short-term culture. Indeed, several stable DC lines and clones have been established from various tissues of mice and humans, providing useful experimental tools for studying the biology of DCs at both molecular and biochemical levels and for the establishment of new DC-based immunotherapies. In this chapter, we will describe the development of long-term DC lines that maintain the growth factor dependence and their immature functional state, thus providing a unique opportunity to study the mechanisms of the initiation of the immune response to infectious agents.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages17-27
Number of pages11
Volume531
DOIs
Publication statusPublished - 2009

Publication series

NameMethods in Molecular Biology
Volume531
ISSN (Print)10643745

Keywords

  • Bacteria
  • Bone marrow
  • Dendritic cell lines
  • GM-CSF
  • Host-parasite interaction
  • Immune response

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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