Generation of small mutation in large genomic fragments by homologous recombination: Description of the technique and examples of its use

Marco Tripodi, Sabina Perfumo, Robin Ali, Laura Amicone, Cathy Abbott, Riccardo Cortese

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We have developed a technique of homologous recombination in bacteria which allows the mutagenesis of large genomic fragments cloned in cosmids. The desired mutation is first introduced into a plasmid clone and is then transferred to the appropriate cosmid clone by the means of double antibiotic selection coupled with phenotypic selection. We describe three different types of construct made by this technique.

Original languageEnglish
Pages (from-to)6247-6251
Number of pages5
JournalNucleic Acids Research
Volume18
Issue number21
Publication statusPublished - Nov 11 1990

Fingerprint

Cosmids
Mutagenesis
Homologous Recombination
Antibiotics
Clone
Recombination
Genomics
Bacteria
Fragment
Mutation
Clone Cells
Plasmids
Anti-Bacterial Agents

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Generation of small mutation in large genomic fragments by homologous recombination : Description of the technique and examples of its use. / Tripodi, Marco; Perfumo, Sabina; Ali, Robin; Amicone, Laura; Abbott, Cathy; Cortese, Riccardo.

In: Nucleic Acids Research, Vol. 18, No. 21, 11.11.1990, p. 6247-6251.

Research output: Contribution to journalArticle

Tripodi, Marco ; Perfumo, Sabina ; Ali, Robin ; Amicone, Laura ; Abbott, Cathy ; Cortese, Riccardo. / Generation of small mutation in large genomic fragments by homologous recombination : Description of the technique and examples of its use. In: Nucleic Acids Research. 1990 ; Vol. 18, No. 21. pp. 6247-6251.
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