Generation of the human induced pluripotent stem cell (hiPSC) line PSMi005-A from a patient carrying the KCNQ1-R190W mutation

Manuela Mura, Yee Ki Lee, Federica Pisano, Monia Ginevrino, Marina Boni, Federica Calabrò, Lia Crotti, Enza Maria Valente, Peter J. Schwartz, Hung Fat Tse, Massimiliano Gnecchi

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Abstract

We generated human induced pluripotent stem cells (hiPSCs) from dermal fibroblasts of a woman carrier of the heterozygous mutation c.568C > T p.R190W on the KCNQ1 gene. hiPSCs, obtained using four retroviruses enconding the reprogramming factors OCT4, SOX2, cMYC and KLF4, display pluripotent stem cell characteristics, and can be differentiated into spontaneously beating cardiomyocytes (hiPSC-CMs).

Original languageEnglish
Article number101437
JournalStem Cell Research
Volume37
DOIs
Publication statusPublished - May 1 2019

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Induced Pluripotent Stem Cells
Cell Line
Mutation
Pluripotent Stem Cells
Retroviridae
Cardiac Myocytes
Fibroblasts
Skin
Genes

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

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title = "Generation of the human induced pluripotent stem cell (hiPSC) line PSMi005-A from a patient carrying the KCNQ1-R190W mutation",
abstract = "We generated human induced pluripotent stem cells (hiPSCs) from dermal fibroblasts of a woman carrier of the heterozygous mutation c.568C > T p.R190W on the KCNQ1 gene. hiPSCs, obtained using four retroviruses enconding the reprogramming factors OCT4, SOX2, cMYC and KLF4, display pluripotent stem cell characteristics, and can be differentiated into spontaneously beating cardiomyocytes (hiPSC-CMs).",
author = "Manuela Mura and Lee, {Yee Ki} and Federica Pisano and Monia Ginevrino and Marina Boni and Federica Calabr{\`o} and Lia Crotti and Valente, {Enza Maria} and Schwartz, {Peter J.} and Tse, {Hung Fat} and Massimiliano Gnecchi",
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AU - Mura, Manuela

AU - Lee, Yee Ki

AU - Pisano, Federica

AU - Ginevrino, Monia

AU - Boni, Marina

AU - Calabrò, Federica

AU - Crotti, Lia

AU - Valente, Enza Maria

AU - Schwartz, Peter J.

AU - Tse, Hung Fat

AU - Gnecchi, Massimiliano

PY - 2019/5/1

Y1 - 2019/5/1

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AB - We generated human induced pluripotent stem cells (hiPSCs) from dermal fibroblasts of a woman carrier of the heterozygous mutation c.568C > T p.R190W on the KCNQ1 gene. hiPSCs, obtained using four retroviruses enconding the reprogramming factors OCT4, SOX2, cMYC and KLF4, display pluripotent stem cell characteristics, and can be differentiated into spontaneously beating cardiomyocytes (hiPSC-CMs).

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