Genes and miRNA expression signatures in peripheral blood mononuclear cells in healthy subjects and patients with metabolic syndrome after acute intake of extra virgin olive oil

Simona D'Amore, Michele Vacca, Marica Cariello, Giusi Graziano, Andria D'Orazio, Roberto Salvia, Rosa Cinzia Sasso, Carlo Sabbà, Giuseppe Palasciano, Antonio Moschetta

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Extra virgin olive oil (EVOO) consumption has been associated with reduced cardiovascular risk but molecular mechanisms underlying its beneficial effects are not fully understood. Here we aimed to identify genes and miRNAs expression changes mediated by acute high- and low-polyphenols EVOO intake. Pre- and post-challenge gene and miRNAs expression analysis was performed on the peripheral blood mononuclear cells (PBMCs) of 12 healthy subjects and 12 patients with metabolic syndrome (MS) by using microarray and RT-qPCR. In healthy subjects, acute intake of EVOO rich in polyphenols was able to ameliorate glycaemia and insulin sensitivity, and to modulate the transcription of genes and miRNAs involved in metabolism, inflammation and cancer, switching PBMCs to a less deleterious inflammatory phenotype; weaker effects were observed in patients with MS as well as in healthy subjects following low-polyphenol EVOO challenge. Concluding, our study shows that acute high-polyphenols EVOO intake is able to modify the transcriptome of PBMCs through the modulation of different pathways associated with the pathophysiology of cardio-metabolic disease and cancer. These beneficial effects are maximized in healthy subjects, and by the use of EVOO cultivars rich in polyphenols. Nutrigenomic changes induced by EVOO thus legitimate the well-known beneficial effects of EVOO in promoting human health and, potentially, preventing the onset of cardiovascular disease and cancer.

Original languageEnglish
Pages (from-to)1671-1680
Number of pages10
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume1861
Issue number11
DOIs
Publication statusPublished - Nov 1 2016

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MicroRNAs
Transcriptome
Blood Cells
Healthy Volunteers
Polyphenols
Nutrigenomics
Gene Expression
Neoplasms
Olive Oil
Metabolic Diseases
Insulin Resistance
Cardiovascular Diseases
Inflammation
Phenotype
Health
Genes

Keywords

  • Extra virgin olive oil
  • Gene expression
  • miRNAs
  • PBMCs

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Genes and miRNA expression signatures in peripheral blood mononuclear cells in healthy subjects and patients with metabolic syndrome after acute intake of extra virgin olive oil. / D'Amore, Simona; Vacca, Michele; Cariello, Marica; Graziano, Giusi; D'Orazio, Andria; Salvia, Roberto; Sasso, Rosa Cinzia; Sabbà, Carlo; Palasciano, Giuseppe; Moschetta, Antonio.

In: Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, Vol. 1861, No. 11, 01.11.2016, p. 1671-1680.

Research output: Contribution to journalArticle

D'Amore, Simona ; Vacca, Michele ; Cariello, Marica ; Graziano, Giusi ; D'Orazio, Andria ; Salvia, Roberto ; Sasso, Rosa Cinzia ; Sabbà, Carlo ; Palasciano, Giuseppe ; Moschetta, Antonio. / Genes and miRNA expression signatures in peripheral blood mononuclear cells in healthy subjects and patients with metabolic syndrome after acute intake of extra virgin olive oil. In: Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids. 2016 ; Vol. 1861, No. 11. pp. 1671-1680.
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