Abstract
To obtain further information about the ontogeny of the T-cell antigen/MHC receptor, a Ti β subunit cDNA probe and heteroantisera specific for the Ti α and Ti β subunits were utilized to characterize human T-lineage cells. Analysis of thymic tumors and normal thymocytes at both the DNA and protein levels demonstrates that Ti β gene rearrangement is evident in stage II (T11+T6+T3-) and stage III (T11+T6-T3+), but not stage I (T11+T6-T3-) thymocytes. In contrast, surface expression of Ti α and Ti β molecules is exclusively restricted to stage III thymocytes. Thus human T-lineage ontogeny is characterized by an orderly series of differentiation steps wherein Ti β gene rearrangement precedes surface expression of the T3-Ti molecular complex.
| Original language | English |
|---|---|
| Pages (from-to) | 261-266 |
| Number of pages | 6 |
| Journal | Cell |
| Volume | 39 |
| Issue number | 2 PART 1 |
| DOIs | |
| Publication status | Published - 1984 |
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ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
- Medicine(all)
Cite this
Genes encoding the Ti β subunit of the antigen/MHC receptor undergo rearrangement during intrathymic ontogeny prior to surface T3-Ti expression. / Royer, Hans Dieter; Acuto, Oreste; Fabbi, Marina; Tizard, Richard; Ramachandran, Kuzhalmannam; Smart, John E.; Reinherz, Ellis L.
In: Cell, Vol. 39, No. 2 PART 1, 1984, p. 261-266.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genes encoding the Ti β subunit of the antigen/MHC receptor undergo rearrangement during intrathymic ontogeny prior to surface T3-Ti expression
AU - Royer, Hans Dieter
AU - Acuto, Oreste
AU - Fabbi, Marina
AU - Tizard, Richard
AU - Ramachandran, Kuzhalmannam
AU - Smart, John E.
AU - Reinherz, Ellis L.
PY - 1984
Y1 - 1984
N2 - To obtain further information about the ontogeny of the T-cell antigen/MHC receptor, a Ti β subunit cDNA probe and heteroantisera specific for the Ti α and Ti β subunits were utilized to characterize human T-lineage cells. Analysis of thymic tumors and normal thymocytes at both the DNA and protein levels demonstrates that Ti β gene rearrangement is evident in stage II (T11+T6+T3-) and stage III (T11+T6-T3+), but not stage I (T11+T6-T3-) thymocytes. In contrast, surface expression of Ti α and Ti β molecules is exclusively restricted to stage III thymocytes. Thus human T-lineage ontogeny is characterized by an orderly series of differentiation steps wherein Ti β gene rearrangement precedes surface expression of the T3-Ti molecular complex.
AB - To obtain further information about the ontogeny of the T-cell antigen/MHC receptor, a Ti β subunit cDNA probe and heteroantisera specific for the Ti α and Ti β subunits were utilized to characterize human T-lineage cells. Analysis of thymic tumors and normal thymocytes at both the DNA and protein levels demonstrates that Ti β gene rearrangement is evident in stage II (T11+T6+T3-) and stage III (T11+T6-T3+), but not stage I (T11+T6-T3-) thymocytes. In contrast, surface expression of Ti α and Ti β molecules is exclusively restricted to stage III thymocytes. Thus human T-lineage ontogeny is characterized by an orderly series of differentiation steps wherein Ti β gene rearrangement precedes surface expression of the T3-Ti molecular complex.
UR - http://www.scopus.com/inward/record.url?scp=0021716154&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021716154&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(84)90003-5
DO - 10.1016/0092-8674(84)90003-5
M3 - Article
C2 - 6333925
AN - SCOPUS:0021716154
VL - 39
SP - 261
EP - 266
JO - Cell
JF - Cell
SN - 0092-8674
IS - 2 PART 1
ER -