Genes encoding the Ti β subunit of the antigen/MHC receptor undergo rearrangement during intrathymic ontogeny prior to surface T3-Ti expression

Hans Dieter Royer, Oreste Acuto, Marina Fabbi, Richard Tizard, Kuzhalmannam Ramachandran, John E. Smart, Ellis L. Reinherz

Research output: Contribution to journalArticle

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Abstract

To obtain further information about the ontogeny of the T-cell antigen/MHC receptor, a Ti β subunit cDNA probe and heteroantisera specific for the Ti α and Ti β subunits were utilized to characterize human T-lineage cells. Analysis of thymic tumors and normal thymocytes at both the DNA and protein levels demonstrates that Ti β gene rearrangement is evident in stage II (T11+T6+T3-) and stage III (T11+T6-T3+), but not stage I (T11+T6-T3-) thymocytes. In contrast, surface expression of Ti α and Ti β molecules is exclusively restricted to stage III thymocytes. Thus human T-lineage ontogeny is characterized by an orderly series of differentiation steps wherein Ti β gene rearrangement precedes surface expression of the T3-Ti molecular complex.

Original languageEnglish
Pages (from-to)261-266
Number of pages6
JournalCell
Volume39
Issue number2 PART 1
DOIs
Publication statusPublished - 1984

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Antigen Receptors
Gene encoding
Thymocytes
Gene Rearrangement
Genes
Antigens
T-cells
Thymus Neoplasms
Tumors
Complementary DNA
T-Cell Antigen Receptor
Molecules
DNA
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Genes encoding the Ti β subunit of the antigen/MHC receptor undergo rearrangement during intrathymic ontogeny prior to surface T3-Ti expression. / Royer, Hans Dieter; Acuto, Oreste; Fabbi, Marina; Tizard, Richard; Ramachandran, Kuzhalmannam; Smart, John E.; Reinherz, Ellis L.

In: Cell, Vol. 39, No. 2 PART 1, 1984, p. 261-266.

Research output: Contribution to journalArticle

Royer, Hans Dieter ; Acuto, Oreste ; Fabbi, Marina ; Tizard, Richard ; Ramachandran, Kuzhalmannam ; Smart, John E. ; Reinherz, Ellis L. / Genes encoding the Ti β subunit of the antigen/MHC receptor undergo rearrangement during intrathymic ontogeny prior to surface T3-Ti expression. In: Cell. 1984 ; Vol. 39, No. 2 PART 1. pp. 261-266.
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AU - Ramachandran, Kuzhalmannam

AU - Smart, John E.

AU - Reinherz, Ellis L.

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