Genes encoding the Ti β subunit of the antigen/MHC receptor undergo rearrangement during intrathymic ontogeny prior to surface T3-Ti expression

Hans Dieter Royer, Oreste Acuto, Marina Fabbi, Richard Tizard, Kuzhalmannam Ramachandran, John E. Smart, Ellis L. Reinherz

Research output: Contribution to journalArticlepeer-review

Abstract

To obtain further information about the ontogeny of the T-cell antigen/MHC receptor, a Ti β subunit cDNA probe and heteroantisera specific for the Ti α and Ti β subunits were utilized to characterize human T-lineage cells. Analysis of thymic tumors and normal thymocytes at both the DNA and protein levels demonstrates that Ti β gene rearrangement is evident in stage II (T11+T6+T3-) and stage III (T11+T6-T3+), but not stage I (T11+T6-T3-) thymocytes. In contrast, surface expression of Ti α and Ti β molecules is exclusively restricted to stage III thymocytes. Thus human T-lineage ontogeny is characterized by an orderly series of differentiation steps wherein Ti β gene rearrangement precedes surface expression of the T3-Ti molecular complex.

Original languageEnglish
Pages (from-to)261-266
Number of pages6
JournalCell
Volume39
Issue number2 PART 1
DOIs
Publication statusPublished - 1984

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

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