Genetic and biochemical characterization of TRU-1, the endogenous class C β-lactamase from Aeromonas enteropelogenes

Filomena De Luca, Chantal Giraud-Morin, Gian Maria Rossolini, Jean Denis Docquier, Thierry Fosse

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Abstract

Aeromonas enteropelogenes (formerly A. tructi) was described to be an ampicillin-susceptible and cephalothinresistant Aeromonas species, which suggests the production of a cephalosporinase. Strain ATCC 49803 was susceptible to amoxicillin, cefotaxime, and imipenem but resistant to cefazolin (MICs of 2, 0.032, 0.125, and >256 μg/ml, respectively) and produced an inducible β-lactamase. Cefotaxime-resistant mutants (MIC, 32 μg/ml) that showed constitutive β-lactamase production could be selected in vitro. The gene coding for the cephalosporinase of A. enteropelogenes ATCC 49803 was cloned, and its biochemical properties were investigated. Escherichia coli transformants showing resistance to various β-lactams carried a 3.5-kb plasmid insert whose sequence revealed a 1,146-bp open reading frame (ORF) encoding a class C β-lactamase, named TRU-1, showing the highest identity scores with A. punctata CAV-1 (75%), A. salmonicida AmpC (75%), and A. hydrophila CepH (71%). The blaTRU-1 locus includes open reading frames (ORFs) showing significant homology with genes found in the genomes of other Aeromonas species, although it exhibits a different organization, as reflected by the presence of additional ORFs located downstream of the β-lactamase gene in the A. hydrophila and A. salmonicida genomes. Specific PCR assays were negative for cphA-like and blaOXA-12-like genes in three A. enteropelogenes ATCC strains. Purified TRU-1 showed a broad substrate profile, efficiently hydrolyzing benzylpenicillin, cephalothin, cefoxitin, and, although with significantly lower turnover rates, oxyiminocephalosporins. Cephaloridine and cefepime were poorly recognized by the enzyme, as reflected by the high K m values observed with these substrates. Thus far, A. enteropelogenes represents the only known example of an Aeromonas species that produces only one β-lactamase belonging to molecular class C.

Original languageEnglish
Pages (from-to)1547-1554
Number of pages8
JournalAntimicrobial Agents and Chemotherapy
Volume54
Issue number4
DOIs
Publication statusPublished - Apr 2010

Fingerprint

Aeromonas
Molecular Biology
Cephalosporinase
Open Reading Frames
Cefotaxime
Genes
Cephaloridine
Genome
Cephalothin
Cefoxitin
Cefazolin
Lactams
Penicillin G
Imipenem
Amoxicillin
Ampicillin
Plasmids
Escherichia coli
Polymerase Chain Reaction
Enzymes

ASJC Scopus subject areas

  • Infectious Diseases
  • Pharmacology (medical)
  • Pharmacology

Cite this

Genetic and biochemical characterization of TRU-1, the endogenous class C β-lactamase from Aeromonas enteropelogenes. / De Luca, Filomena; Giraud-Morin, Chantal; Rossolini, Gian Maria; Docquier, Jean Denis; Fosse, Thierry.

In: Antimicrobial Agents and Chemotherapy, Vol. 54, No. 4, 04.2010, p. 1547-1554.

Research output: Contribution to journalArticle

De Luca, Filomena ; Giraud-Morin, Chantal ; Rossolini, Gian Maria ; Docquier, Jean Denis ; Fosse, Thierry. / Genetic and biochemical characterization of TRU-1, the endogenous class C β-lactamase from Aeromonas enteropelogenes. In: Antimicrobial Agents and Chemotherapy. 2010 ; Vol. 54, No. 4. pp. 1547-1554.
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