TY - JOUR
T1 - Genetic and molecular alterations in rhabdomyosarcoma
T2 - mRNA overexpression of MCL1 and MAP2K4 genes
AU - Pazzaglia, Laura
AU - Chiechi, Antonella
AU - Conti, Amalia
AU - Gamberi, Gabriella
AU - Magagnoli, Giovanna
AU - Novello, Chiara
AU - Morandi, Luca
AU - Picci, Piero
AU - Mercuri, Mario
AU - Benassi, Maria Serena
PY - 2009
Y1 - 2009
N2 - Rhabdomyosarcoma, the most common soft tissue sarcoma in childhood, belongs to the small round cell tumor family and is classified according to its histopathological features as embryonal, alveolar and pleomorphic. In this study we propose to explore genetic alterations involved in rhabdomyosarcoma tumorigenesis and assess the level of mRNA gene expression of controlling survival signalling pathways. For genetic and molecular analysis, array-based comparative genomic hybridization, combined with Real Time PCR using the comparative method, was performed on 14 primary well-characterized human primary rhabdomyosarcomas. Multiple changeg affecting chromosome arms were detected in all cases, including gain or loss of specific regions harbouring cancer progression-associated genes. Evaluation of mRNA levels showed in the majority of cases overexpression of MCL1 and MAP2K4 genes, both involved in cell viability regulation. Our findings on rhabdomyosarcoma samples showed multiple copy number alterations in chromosome regions implicated in malignancy progression and indicated a strong expression of MAP2K4 and MCL1 genes, both involved in different biological functions of complicated signalling pathways.
AB - Rhabdomyosarcoma, the most common soft tissue sarcoma in childhood, belongs to the small round cell tumor family and is classified according to its histopathological features as embryonal, alveolar and pleomorphic. In this study we propose to explore genetic alterations involved in rhabdomyosarcoma tumorigenesis and assess the level of mRNA gene expression of controlling survival signalling pathways. For genetic and molecular analysis, array-based comparative genomic hybridization, combined with Real Time PCR using the comparative method, was performed on 14 primary well-characterized human primary rhabdomyosarcomas. Multiple changeg affecting chromosome arms were detected in all cases, including gain or loss of specific regions harbouring cancer progression-associated genes. Evaluation of mRNA levels showed in the majority of cases overexpression of MCL1 and MAP2K4 genes, both involved in cell viability regulation. Our findings on rhabdomyosarcoma samples showed multiple copy number alterations in chromosome regions implicated in malignancy progression and indicated a strong expression of MAP2K4 and MCL1 genes, both involved in different biological functions of complicated signalling pathways.
KW - CGH-microarray
KW - Gene expression
KW - Real-Time PCR
KW - Rhabdomyosarcoma
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M3 - Article
C2 - 19012245
AN - SCOPUS:60549112596
VL - 24
SP - 61
EP - 67
JO - Histology and Histopathology
JF - Histology and Histopathology
SN - 0213-3911
IS - 1
ER -