Abstract
Intra-host evolution of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) has been shown by viral RNA analysis in subjects who naturally suppress plasma viremia to low levels, known as controllers. However, little is known about the variability of proviral DNA and the inter-relationships among contained systemic viremia, rate of reservoir reseeding and specific major histocompatibility complex (MHC) genotypes, in controllers. Here, we analysed the proviral DNA quasispecies of the env V1-V2 region, in PBMCs and in anatomical compartments of 13 long-term controller monkeys after 3.2 years of infection with simian/human immunodeficiency virus (SHIV)SF162P4cy. A considerable variation in the genetic diversity of proviral quasispecies was present among animals. Seven monkeys exhibited env V1-V2 proviral populations composed of both clusters of identical ancestral sequences and new variants, whereas the other six monkeys displayed relatively high env V1-V2 genetic diversity with a large proportion of diverse novel sequences. Our results demonstrate that in SHIVSF162P4cy-infected monkeys there exists a disparate pattern of intra-host viral diversity and that reseeding of the proviral reservoir occurs in some animals. Moreover, even though no particular association has been observed between MHC haplotypes and the long-term control of infection, a remarkably similar pattern of intra-host viral diversity and divergence was found within animals carrying the M3 haplotype. This suggests that in animals bearing the same MHC haplotype and infected with the same virus, viral diversity follows a similar pattern with similar outcomes and control of infection.
| Original language | English |
|---|---|
| Article number | 001159 |
| Pages (from-to) | 1717-1728 |
| Number of pages | 12 |
| Journal | Journal of General Virology |
| Volume | 99 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - Dec 1 2018 |
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Keywords
- Diversity
- Evolution
- MCM
- MHC
- SHIV
- Viremic controllers
ASJC Scopus subject areas
- Virology
Cite this
Genetic diversity in the env V1-V2 region of proviral quasispecies from long-term controller MHC-typed cynomolgus macaques infected with SHIVSF162P4cy. / Capone, Alessia; Lo Presti, Alessandra; Sernicola, Leonardo; Farcomeni, Stefania; Ferrantelli, Flavia; Maggiorella, Maria T.; Mee, Edward T.; Rose, Nicola J.; Cella, Eleonora; Ciccozzi, Massimo; Ensoli, Barbara; Borsetti, Alessandra.
In: Journal of General Virology, Vol. 99, No. 12, 001159, 01.12.2018, p. 1717-1728.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genetic diversity in the env V1-V2 region of proviral quasispecies from long-term controller MHC-typed cynomolgus macaques infected with SHIVSF162P4cy
AU - Capone, Alessia
AU - Lo Presti, Alessandra
AU - Sernicola, Leonardo
AU - Farcomeni, Stefania
AU - Ferrantelli, Flavia
AU - Maggiorella, Maria T.
AU - Mee, Edward T.
AU - Rose, Nicola J.
AU - Cella, Eleonora
AU - Ciccozzi, Massimo
AU - Ensoli, Barbara
AU - Borsetti, Alessandra
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Intra-host evolution of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) has been shown by viral RNA analysis in subjects who naturally suppress plasma viremia to low levels, known as controllers. However, little is known about the variability of proviral DNA and the inter-relationships among contained systemic viremia, rate of reservoir reseeding and specific major histocompatibility complex (MHC) genotypes, in controllers. Here, we analysed the proviral DNA quasispecies of the env V1-V2 region, in PBMCs and in anatomical compartments of 13 long-term controller monkeys after 3.2 years of infection with simian/human immunodeficiency virus (SHIV)SF162P4cy. A considerable variation in the genetic diversity of proviral quasispecies was present among animals. Seven monkeys exhibited env V1-V2 proviral populations composed of both clusters of identical ancestral sequences and new variants, whereas the other six monkeys displayed relatively high env V1-V2 genetic diversity with a large proportion of diverse novel sequences. Our results demonstrate that in SHIVSF162P4cy-infected monkeys there exists a disparate pattern of intra-host viral diversity and that reseeding of the proviral reservoir occurs in some animals. Moreover, even though no particular association has been observed between MHC haplotypes and the long-term control of infection, a remarkably similar pattern of intra-host viral diversity and divergence was found within animals carrying the M3 haplotype. This suggests that in animals bearing the same MHC haplotype and infected with the same virus, viral diversity follows a similar pattern with similar outcomes and control of infection.
AB - Intra-host evolution of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) has been shown by viral RNA analysis in subjects who naturally suppress plasma viremia to low levels, known as controllers. However, little is known about the variability of proviral DNA and the inter-relationships among contained systemic viremia, rate of reservoir reseeding and specific major histocompatibility complex (MHC) genotypes, in controllers. Here, we analysed the proviral DNA quasispecies of the env V1-V2 region, in PBMCs and in anatomical compartments of 13 long-term controller monkeys after 3.2 years of infection with simian/human immunodeficiency virus (SHIV)SF162P4cy. A considerable variation in the genetic diversity of proviral quasispecies was present among animals. Seven monkeys exhibited env V1-V2 proviral populations composed of both clusters of identical ancestral sequences and new variants, whereas the other six monkeys displayed relatively high env V1-V2 genetic diversity with a large proportion of diverse novel sequences. Our results demonstrate that in SHIVSF162P4cy-infected monkeys there exists a disparate pattern of intra-host viral diversity and that reseeding of the proviral reservoir occurs in some animals. Moreover, even though no particular association has been observed between MHC haplotypes and the long-term control of infection, a remarkably similar pattern of intra-host viral diversity and divergence was found within animals carrying the M3 haplotype. This suggests that in animals bearing the same MHC haplotype and infected with the same virus, viral diversity follows a similar pattern with similar outcomes and control of infection.
KW - Diversity
KW - Evolution
KW - MCM
KW - MHC
KW - SHIV
KW - Viremic controllers
UR - http://www.scopus.com/inward/record.url?scp=85059660768&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85059660768&partnerID=8YFLogxK
U2 - 10.1099/jgv.0.001159
DO - 10.1099/jgv.0.001159
M3 - Article
AN - SCOPUS:85059660768
VL - 99
SP - 1717
EP - 1728
JO - Journal of General Virology
JF - Journal of General Virology
SN - 0022-1317
IS - 12
M1 - 001159
ER -